Cloned (Comment) | Organism |
---|---|
chromosome-encoded metallo-beta-lactamase, genotyping in Aliivibrio isolates, recombinant enzyme overexpression in Escherichia coli | Aliivibrio salmonicida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Aliivibrio salmonicida | |
DTT | - |
Aliivibrio salmonicida | |
TCEP | complete inhibition | Aliivibrio salmonicida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
nitrocefin | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.017 | - |
ertapenem | pH 7.2, 37°C, recombinant enzyme, in presence of NaCl | Aliivibrio salmonicida | |
0.037 | - |
ceftazidime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.068 | - |
cefuroxime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.073 | - |
ertapenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.079 | - |
cefoxitin | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.142 | - |
meropenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.177 | - |
cefepime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.933 | - |
Imipenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Aliivibrio salmonicida | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 40% activity increase at 0.5 M | Aliivibrio salmonicida | |
Zn2+ | a zinc enzyme | Aliivibrio salmonicida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aliivibrio salmonicida | the enzyme hydrolyses all beta-lactam antibiotics tested, except for the monobactam aztreonam and the penicillin piperacillin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio salmonicida | B6EPH0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Aliivibrio salmonicida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cefepime + H2O | - |
Aliivibrio salmonicida | ? | - |
? | |
cefoxitin + H2O | - |
Aliivibrio salmonicida | ? | - |
? | |
ceftazidime + H2O | - |
Aliivibrio salmonicida | ? | - |
? | |
cefuroxime + H2O | - |
Aliivibrio salmonicida | ? | - |
? | |
ertapenem + H2O | - |
Aliivibrio salmonicida | ? | - |
? | |
imipenem + H2O | - |
Aliivibrio salmonicida | ? | - |
? | |
meropenem + H2O | - |
Aliivibrio salmonicida | ? | - |
? | |
additional information | the enzyme hydrolyses all beta-lactam antibiotics tested, except for the monobactam aztreonam and the penicillin piperacillin | Aliivibrio salmonicida | ? | - |
? | |
additional information | no activity with piperacillin and aztreonam | Aliivibrio salmonicida | ? | - |
? | |
nitrocefin + H2O | nitrocefin shows autohydrolysis at pH 7.5-9.0 in Tris buffer, while not in HEPES buffer with the same pH | Aliivibrio salmonicida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALI-1 | - |
Aliivibrio salmonicida |
MBL | - |
Aliivibrio salmonicida |
metallo-beta-lactamase | - |
Aliivibrio salmonicida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Aliivibrio salmonicida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature dependency at different NaCl concentrations, overview | Aliivibrio salmonicida |
- |
50 | activity range, profile, overview | Aliivibrio salmonicida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
ceftazidime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.27 | - |
cefoxitin | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
0.58 | - |
cefepime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
4 | - |
ertapenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
4.3 | - |
ertapenem | pH 7.2, 37°C, recombinant enzyme, in presence of NaCl | Aliivibrio salmonicida | |
6.3 | - |
nitrocefin | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
8 | - |
Imipenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
11 | - |
cefuroxime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
13 | - |
meropenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.3 | - |
Aliivibrio salmonicida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
broad pH range | Aliivibrio salmonicida |
Organism | Comment | Expression |
---|---|---|
Aliivibrio salmonicida | the marine enzyme is highly salt tolerant and cold active | additional information |
General Information | Comment | Organism |
---|---|---|
evolution | metallo-beta-lactamases (MBLs) belong to the protein family of beta-lactamases, a group of enzymes that deactivate beta-lactam antibiotics by cleaving the amide bond in the beta-lactam ring, the enzyme belongs to the molecular class B, subclass B1. The Ambler class B beta-lactamase shares 39% and 29% amino acid identity with IMP-1 and VIM-2, from a Pseudomonas aeruginosa hospital isolate, respectively. Active-site residues that are coordinating zinc ions are conserved among all three subclasses | Aliivibrio salmonicida |
additional information | the marine enzyme is highly salt tolerant and cold active | Aliivibrio salmonicida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
ceftazidime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
3.3 | - |
cefepime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
3.4 | - |
cefoxitin | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
8.5 | - |
Imipenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
54 | - |
ertapenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
91.6 | - |
meropenem | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
160 | - |
cefuroxime | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida | |
250 | - |
ertapenem | pH 7.2, 37°C, recombinant enzyme, in presence of NaCl | Aliivibrio salmonicida | |
1000 | - |
nitrocefin | pH 7.2, 37°C, recombinant enzyme | Aliivibrio salmonicida |