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Literature summary for 3.5.2.6 extracted from

  • Kristiansen, A.; Grgic, M.; Altermark, B.; Leiros, I.
    Properties and distribution of a metallo-beta-lactamase (ALI-1) from the fish pathogen Aliivibrio salmonicida LFI1238 (2015), J. Antimicrob. Chemother., 70, 766-772.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
chromosome-encoded metallo-beta-lactamase, genotyping in Aliivibrio isolates, recombinant enzyme overexpression in Escherichia coli Aliivibrio salmonicida

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Aliivibrio salmonicida
DTT
-
Aliivibrio salmonicida
TCEP complete inhibition Aliivibrio salmonicida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
nitrocefin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.017
-
ertapenem pH 7.2, 37°C, recombinant enzyme, in presence of NaCl Aliivibrio salmonicida
0.037
-
ceftazidime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.068
-
cefuroxime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.073
-
ertapenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.079
-
cefoxitin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.142
-
meropenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.177
-
cefepime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.933
-
Imipenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Aliivibrio salmonicida
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 40% activity increase at 0.5 M Aliivibrio salmonicida
Zn2+ a zinc enzyme Aliivibrio salmonicida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aliivibrio salmonicida the enzyme hydrolyses all beta-lactam antibiotics tested, except for the monobactam aztreonam and the penicillin piperacillin ?
-
?

Organism

Organism UniProt Comment Textmining
Aliivibrio salmonicida B6EPH0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Aliivibrio salmonicida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cefepime + H2O
-
Aliivibrio salmonicida ?
-
?
cefoxitin + H2O
-
Aliivibrio salmonicida ?
-
?
ceftazidime + H2O
-
Aliivibrio salmonicida ?
-
?
cefuroxime + H2O
-
Aliivibrio salmonicida ?
-
?
ertapenem + H2O
-
Aliivibrio salmonicida ?
-
?
imipenem + H2O
-
Aliivibrio salmonicida ?
-
?
meropenem + H2O
-
Aliivibrio salmonicida ?
-
?
additional information the enzyme hydrolyses all beta-lactam antibiotics tested, except for the monobactam aztreonam and the penicillin piperacillin Aliivibrio salmonicida ?
-
?
additional information no activity with piperacillin and aztreonam Aliivibrio salmonicida ?
-
?
nitrocefin + H2O nitrocefin shows autohydrolysis at pH 7.5-9.0 in Tris buffer, while not in HEPES buffer with the same pH Aliivibrio salmonicida ?
-
?

Synonyms

Synonyms Comment Organism
ALI-1
-
Aliivibrio salmonicida
MBL
-
Aliivibrio salmonicida
metallo-beta-lactamase
-
Aliivibrio salmonicida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Aliivibrio salmonicida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
temperature dependency at different NaCl concentrations, overview Aliivibrio salmonicida
-
50 activity range, profile, overview Aliivibrio salmonicida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
ceftazidime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.27
-
cefoxitin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
0.58
-
cefepime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
4
-
ertapenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
4.3
-
ertapenem pH 7.2, 37°C, recombinant enzyme, in presence of NaCl Aliivibrio salmonicida
6.3
-
nitrocefin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
8
-
Imipenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
11
-
cefuroxime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
13
-
meropenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.3
-
Aliivibrio salmonicida

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
broad pH range Aliivibrio salmonicida

Expression

Organism Comment Expression
Aliivibrio salmonicida the marine enzyme is highly salt tolerant and cold active additional information

General Information

General Information Comment Organism
evolution metallo-beta-lactamases (MBLs) belong to the protein family of beta-lactamases, a group of enzymes that deactivate beta-lactam antibiotics by cleaving the amide bond in the beta-lactam ring, the enzyme belongs to the molecular class B, subclass B1. The Ambler class B beta-lactamase shares 39% and 29% amino acid identity with IMP-1 and VIM-2, from a Pseudomonas aeruginosa hospital isolate, respectively. Active-site residues that are coordinating zinc ions are conserved among all three subclasses Aliivibrio salmonicida
additional information the marine enzyme is highly salt tolerant and cold active Aliivibrio salmonicida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4
-
ceftazidime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.3
-
cefepime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.4
-
cefoxitin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
8.5
-
Imipenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
54
-
ertapenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
91.6
-
meropenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
160
-
cefuroxime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
250
-
ertapenem pH 7.2, 37°C, recombinant enzyme, in presence of NaCl Aliivibrio salmonicida
1000
-
nitrocefin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida