Cloned (Comment) | Organism |
---|---|
gene blaVIM-2, recombinant Co2+-enzyme expression in Escherichia coli strain BL21(DE3) in in minimal medium | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapor diffusion method, mixxing of 0001 ml of 10 mg/mL VIM-2 protein with 0.001 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 mM zinc chloride, 0.1 M HEPES, pH 7.5, and 25% PEG 3350, X-ray diffraction tructure determination and analysis, molecular replacement | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state and steady-state kinetics, overview | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | preparation of a Co(II)-substituted VIM-2 analogue, crystal structure analysis, overview | Pseudomonas aeruginosa | |
additional information | analysis of the metal content of enzyme VIM-2, metal binding kinetics, overview | Pseudomonas aeruginosa | |
Zn2+ | dependent on, crystal structure analysis, overview | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29700 | - |
x * 29700, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9K2N0 | isolated from a clinical strain, gene blaVIM?2 | - |
Purification (Comment) | Organism |
---|---|
recombinant cobalt-cobtaining enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chromacef + H2O | - |
Pseudomonas aeruginosa | (2S)-2-[(S)-carboxy(2-phenylacetamido)methyl]-5-[(E)-2-(4-nitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Pseudomonas aeruginosa | (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29700, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
metallo-beta-lactamase | - |
Pseudomonas aeruginosa |
VIM-2 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing | - |
5.6 |