Any feedback?
Literature summary for 3.5.2.6 extracted from
- Biochemical characterization of the POM-1 metallo-beta-lactamase from Pseudomonas otitidis (2015), Antimicrob. Agents Chemother., 59, 1755-1758.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant overexpression in Escherichia coli strain BL21(DE3) | Pseudomonas otitidis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Pseudomonas otitidis | |
| 0.004 | - |
ertapenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis |  |
| 0.007 | - |
cephalothin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.007 | - |
doripenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.016 | - |
meropenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.023 | - |
ampicillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.052 | - |
Imipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.053 | - |
piperacillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.063 | - |
cefotaxime | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.102 | - |
panipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 0.5 | - |
ticarcillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a dizinc enzyme | Pseudomonas otitidis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28636 | - |
4 * 28636, recombinant enzyme, mass spectrometry, 4 * 28639, sequence calculation | Pseudomonas otitidis |
| 28639 | - |
4 * 28636, recombinant enzyme, mass spectrometry, 4 * 28639, sequence calculation | Pseudomonas otitidis |
| 115000 | - |
recombinant enzyme, gel filtration | Pseudomonas otitidis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas otitidis | E6Y3G9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) by ultrafiltration and anion exchange chromatography | Pseudomonas otitidis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ampicillin + H2O | - |
Pseudomonas otitidis | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cefotaxime + H2O | - |
Pseudomonas otitidis | ? | - |
? | |
| cephalothin + H2O | - |
Pseudomonas otitidis | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| doripenem + H2O | - |
Pseudomonas otitidis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-([(3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| ertapenem + H2O | - |
Pseudomonas otitidis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl]sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| imipenem + H2O | - |
Pseudomonas otitidis | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([2-[(iminomethyl)amino]ethyl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| meropenem + H2O | - |
Pseudomonas otitidis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| additional information | the recombinant enzyme shows broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins. Poor activity with ceftazidime and cefepime | Pseudomonas otitidis | ? | - |
? | |
| panipenem + H2O | - |
Pseudomonas otitidis | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S)-1-ethanimidoylpyrrolidin-3-yl]sulfanyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| piperacillin + H2O | - |
Pseudomonas otitidis | (2R,4S)-2-[(R)-carboxy([(2R)-2-[(4-ethyl-2,3-dioxopiperazine-1-carbonyl)amino]-2-phenylacetyl]amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ticarcillin + H2O | - |
Pseudomonas otitidis | (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 28636, recombinant enzyme, mass spectrometry, 4 * 28639, sequence calculation | Pseudomonas otitidis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| metallo-beta-lactamase | - |
Pseudomonas otitidis |
| POM-1 | - |
Pseudomonas otitidis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas otitidis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
piperacillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 51 | - |
doripenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 56 | - |
cefotaxime | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 60 | - |
cephalothin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 69 | - |
ertapenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 190 | - |
ampicillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 190 | - |
ticarcillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 220 | - |
meropenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 312 | - |
Imipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 500 | - |
panipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the POM-1 metallo-beta-lactamase is a subclass B3 resident enzyme | Pseudomonas otitidis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 170 | - |
piperacillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 390 | - |
ticarcillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 900 | - |
cefotaxime | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 4900 | - |
panipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 6000 | - |
Imipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 6800 | - |
doripenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 8100 | - |
ampicillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 8900 | - |
cephalothin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 14000 | - |
meropenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 15000 | - |
ertapenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
