Protein Variants | Comment | Organism |
---|---|---|
A172E | random mutagenesis | Burkholderia thailandensis |
A172P | random mutagenesis | Burkholderia thailandensis |
A172T | random mutagenesis | Burkholderia thailandensis |
A172V | random mutagenesis | Burkholderia thailandensis |
C69F | random mutagenesis | Burkholderia thailandensis |
C69Y | random mutagenesis | Burkholderia thailandensis |
D176G | random mutagenesis | Burkholderia thailandensis |
D176N | random mutagenesis | Burkholderia thailandensis |
D179N | random mutagenesis | Burkholderia thailandensis |
E166D | random mutagenesis | Burkholderia thailandensis |
E166G | random mutagenesis | Burkholderia thailandensis |
E166K | random mutagenesis | Burkholderia thailandensis |
E168del | random mutagenesis | Burkholderia thailandensis |
L162F | random mutagenesis | Burkholderia thailandensis |
L169P | random mutagenesis | Burkholderia thailandensis |
L169Q | random mutagenesis | Burkholderia thailandensis |
L169R | random mutagenesis | Burkholderia thailandensis |
additional information | large scale mutant screening, construction of four deletion mutations in a class A beta-lactamase PenA, each conferring an extended substrate spectrum. Single-amino-acid deletions T171del, I173del, and P174del and a two-amino-acid deletion, R165_T167delinsP, occurr in the omega loop, increasing the flexibility of the binding cavity. The four additional deletion mutations in PenA extend the substrate spectrum, in addition to the characterized E168del mutation. The mutations are potential future mutations conferring high-level ceftazidime resistance on isolates from clinical settings, compared with amino acid substitution mutations. MIC values of mutant enzymes for antibiotics ceftazidime, amoxicillin, amoxicillin-clavulanic acid, cefotaxime, ceftriaxone, cefepime, and meropenem, overview. None of the deletion mutations confer resistance to cefepime, a fourth-generation cephalosporin, or to meropenem, a carbapenem subgroup member, but the deletion mutations exhibit various profiles of resistance to expanded-spectrum cephalosporins | Burkholderia thailandensis |
N136D | random mutagenesis | Burkholderia thailandensis |
N136K | random mutagenesis | Burkholderia thailandensis |
N136T | random mutagenesis | Burkholderia thailandensis |
N170H | random mutagenesis | Burkholderia thailandensis |
N170K | random mutagenesis | Burkholderia thailandensis |
N170Y | random mutagenesis | Burkholderia thailandensis |
P174del | random mutagenesis | Burkholderia thailandensis |
P174L | random mutagenesis | Burkholderia thailandensis |
P174S | random mutagenesis | Burkholderia thailandensis |
R163S | random mutagenesis | Burkholderia thailandensis |
R164C | random mutagenesis | Burkholderia thailandensis |
R164H | random mutagenesis | Burkholderia thailandensis |
R164L | random mutagenesis | Burkholderia thailandensis |
T171P | random mutagenesis | Burkholderia thailandensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia thailandensis | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
class A beta-lactamase | - |
Burkholderia thailandensis |
PenA | - |
Burkholderia thailandensis |