Cloned (Comment) | Organism |
---|---|
Mox-1 is a unique plasmid-mediated class C beta-lactamase, recombinant expression of His-tagged enzyme in Escherichia coli BL21(DE3)/pLysS | Klebsiella pneumoniae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging-drop or sitting-drop vapor diffusion, mixing of 10 mg/ml protein in 10 mM Tris-HCl, pH 7.0, with reservoir solution containing of 20% PEG 8000, 100 mM sodium cacodylate, pH 6.5, and 0.2 M zinc acetate, to a finaal volume of 0.01 ml, equilibration against 0.5 ml reservoir solution, 1 month, 16°C, X-ray diffraction structure determination and analysis at 1.54 A resolution, modeling and molecular replacement | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aztreonam | - |
Klebsiella pneumoniae | |
additional information | no inhibition by clavulanic acid | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cefepime + H2O | Klebsiella pneumoniae | - |
? | - |
? | |
cefepime + H2O | Klebsiella pneumoniae NU2936 | - |
? | - |
? | |
cephalothin + H2O | Klebsiella pneumoniae | - |
? | - |
? | |
cephalothin + H2O | Klebsiella pneumoniae NU2936 | - |
? | - |
? | |
additional information | Klebsiella pneumoniae | the enzyme hydrolyzes penicillins, cephalothin, and the expanded-spectrum cephalosporins cefepime and moxalactam | ? | - |
? | |
additional information | Klebsiella pneumoniae NU2936 | the enzyme hydrolyzes penicillins, cephalothin, and the expanded-spectrum cephalosporins cefepime and moxalactam | ? | - |
? | |
moxalactam + H2O | Klebsiella pneumoniae | - |
? | - |
? | |
moxalactam + H2O | Klebsiella pneumoniae NU2936 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | Q51578 | mox1, plasmid-encoded | - |
Klebsiella pneumoniae NU2936 | Q51578 | mox1, plasmid-encoded | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli BL21(DE3)/pLysS by nickel affinity chhromatography and gel filtration | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cefepime + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
cefepime + H2O | - |
Klebsiella pneumoniae NU2936 | ? | - |
? | |
cefepime + H2O | - |
Klebsiella pneumoniae | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefepime + H2O | - |
Klebsiella pneumoniae NU2936 | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
cephalothin + H2O | - |
Klebsiella pneumoniae NU2936 | ? | - |
? | |
cephalothin + H2O | - |
Klebsiella pneumoniae | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
additional information | the enzyme hydrolyzes penicillins, cephalothin, and the expanded-spectrum cephalosporins cefepime and moxalactam | Klebsiella pneumoniae | ? | - |
? | |
additional information | the enzyme hydrolyzes penicillins, cephalothin, and the expanded-spectrum cephalosporins cefepime and moxalactam | Klebsiella pneumoniae NU2936 | ? | - |
? | |
moxalactam + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
moxalactam + H2O | - |
Klebsiella pneumoniae NU2936 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure, overview | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
class C beta-lactamase | - |
Klebsiella pneumoniae |
Mox-1 | - |
Klebsiella pneumoniae |
Mox-1 beta-lactamase | - |
Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
additional information | Mox-1 is a unique plasmid-mediated class C beta-lactamase. Structure comparison with other beta-lactamases shows that two region in Mox 1, amino acid residues 214 to 216 positioned in the omega loop and the other in the N-terminus of the B3 beta-strand corresponding to amino acid residues 303 to 306, having significant structural flexibility of these regions, may impact the recognition and binding of substrates in Mox-1 leading to the unique substrate profile of the enzyme, overview. A substrate-induced conformational change underlies the basis of the hydrolytic profile of Mox-1 beta-lactamase, active site structure of enzyme Mox-1, overview | Klebsiella pneumoniae |