Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Klebsiella pneumoniae |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the complex of SHV-1 mutant D104E with beta-lactamse inhibitor protein BLIP, to 1.6 A resolution. Mutation D104E results in a 1000fold enhancement in binding affinity to BLIP, as it restores a salt bridge to BLIP. Mutation of a neighboring residue, BLIP E73M, results in salt bridge formation between SHV-1 D104 and BLIP K74 and a 400fold increase in binding affinity. BLIP F142 cooperatively stabilizes both interactions | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D104E | mutation results in a 1000fold enhancement in binding affinity to beta-lactamase inhibitor protein BLIP, as it restores a salt bridge to BLIP. Mutation of a neighboring residue, BLIP E73M, results in salt bridge formation between SHV-1 D104 and BLIP K74 and a 400fold increase in binding affinity. BLIP F142 cooperatively stabilizes both interactions | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | P0AD64 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
SHV-1 | - |
Klebsiella pneumoniae |