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Literature summary for 3.5.2.6 extracted from
- Purification development and characterization of the zinc-dependent metallo-beta-lactamase from Bacillus anthracis (2011), Biotechnol. Lett., 33, 1417-1422.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Bacillus anthracis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.168 | - |
cephalexin | pH 7.0, 25°C | Bacillus anthracis | |
| 0.776 | - |
penicillin G | pH 7.0, 25°C | Bacillus anthracis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| use of Ni2+-affinity chromatography | Bacillus anthracis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
pH 7.0, 25°C | Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cephalexin + H2O | - |
Bacillus anthracis | (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| penicillin G + H2O | - |
Bacillus anthracis | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bla2 | - |
Bacillus anthracis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
60 min, more than 90% of initial activity | Bacillus anthracis |
| 60 | - |
complete loss of activity after 60 min | Bacillus anthracis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.83 | - |
cephalexin | pH 7.0, 25°C | Bacillus anthracis | |
| 628 | - |
penicillin G | pH 7.0, 25°C | Bacillus anthracis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Bacillus anthracis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Bacillus anthracis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00063 | - |
pH 7.0, 25°C | Bacillus anthracis | EDTA | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.6 | - |
cephalexin | pH 7.0, 25°C | Bacillus anthracis | |
| 809 | - |
penicillin G | pH 7.0, 25°C | Bacillus anthracis | |
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