KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
benzyl [[(2S)-2-hydroxy-4-phenylbutanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
0.071 | - |
benzyl [[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
0.172 | - |
3-[[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]benzoic acid | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
0.32 | - |
3-nitrophenyl (2S)-2-hydroxy-3-phenylpropanoate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
0.34 | - |
benzyl [[(2R)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
0.46 | - |
benzyl [[(2S)-2-hydroxy-2-phenylacetyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
0.52 | - |
benzyl [[(2S)-2-hydroxypropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | P05364 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Enterobacter cloacae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitrophenyl (2S)-2-hydroxy-3-phenylpropanoate + H2O | - |
Enterobacter cloacae | ? | - |
? | |
3-[[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]benzoic acid + H2O | - |
Enterobacter cloacae | ? | - |
? | |
benzyl [[(2R)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate + H2O | - |
Enterobacter cloacae | ? + hydroxamate | - |
? | |
benzyl [[(2S)-2-hydroxy-2-phenylacetyl]oxy]carbamate + H2O | - |
Enterobacter cloacae | ? + hydroxamate | - |
? | |
benzyl [[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate + H2O | - |
Enterobacter cloacae | ? + hydroxamate | - |
? | |
benzyl [[(2S)-2-hydroxy-4-phenylbutanoyl]oxy]carbamate + H2O | - |
Enterobacter cloacae | ? + hydroxamate | - |
? | |
benzyl [[(2S)-2-hydroxypropanoyl]oxy]carbamate + H2O | - |
Enterobacter cloacae | ? + hydroxamate | - |
? | |
additional information | O-(1-carboxy-1-alkyloxycarbonyl) hydroxamates are found to spontaneously decarboxylate in aqueous neutral buffer to form O-(2-hydroxyalkylcarbonyl) hydroxamates.While the former molecules do not react rapidly with serine beta-lactamases, the latter are quite good substrates of representative class A and C, but not D enzymes. The enzymes catalyze hydrolysis of these compounds to a mixture of the R-hydroxy acid and hydroxamate. Although both D- and L-R-hydroxy acid derivatives were substrates, the former are preferred | Enterobacter cloacae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P99 beta-lactamase | - |
Enterobacter cloacae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
benzyl [[(2S)-2-hydroxy-4-phenylbutanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
1.5 | - |
benzyl [[(2S)-2-hydroxypropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
3.5 | - |
benzyl [[(2S)-2-hydroxy-2-phenylacetyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
5.9 | - |
benzyl [[(2R)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
6.9 | - |
3-[[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]benzoic acid | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
8.7 | - |
benzyl [[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
10.9 | - |
3-nitrophenyl (2S)-2-hydroxy-3-phenylpropanoate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
benzyl [[(2S)-2-hydroxypropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
7.5 | - |
benzyl [[(2S)-2-hydroxy-2-phenylacetyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
17.4 | - |
benzyl [[(2R)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
55 | - |
benzyl [[(2S)-2-hydroxy-4-phenylbutanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
69 | - |
3-[[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]benzoic acid | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
109 | - |
3-nitrophenyl (2S)-2-hydroxy-3-phenylpropanoate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae | |
120 | - |
benzyl [[(2S)-2-hydroxy-3-phenylpropanoyl]oxy]carbamate | pH not specified in the publication, temperature not specified in the publication | Enterobacter cloacae |