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Literature summary for 3.5.2.6 extracted from

  • Fonseca, F.; Arthur, C.J.; Bromley, E.H.; Samyn, B.; Moerman, P.; Saavedra, M.J.; Correia, A.; Spencer, J.
    Biochemical characterization of Sfh-I, a subclass B2 metallo-beta-lactamase from Serratia fonticola UTAD54 (2011), Antimicrob. Agents Chemother., 55, 5392-5395.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Serratia fonticola

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ protein binds one equivalent of zinc, no inhibition by a second equivalent of zinc Serratia fonticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0792
-
 Imipenem pH 7.0, 25°C Serratia fonticola
0.091
-
 moxalactam pH 7.0, 25°C Serratia fonticola
0.099
-
 cefoxitin pH 7.0, 25°C Serratia fonticola
0.106
-
 nitrocefin pH 7.0, 25°C Serratia fonticola
0.202
-
 cefepime pH 7.0, 25°C Serratia fonticola
0.215
-
 meropenem pH 7.0, 25°C Serratia fonticola
0.676
-
 ampicillin pH 7.0, 25°C Serratia fonticola
1.28
-
 carbenicillin pH 7.0, 25°C Serratia fonticola

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ protein binds one equivalent of zinc, no inhibition by a second equivalent of zinc Serratia fonticola

Organism

Organism UniProt Comment Textmining
Serratia fonticola Q9RMI1
-
-
Serratia fonticola UTAD54 Q9RMI1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Serratia fonticola (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Serratia fonticola UTAD54 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
carbenicillin + H2O
-
 Serratia fonticola (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
carbenicillin + H2O
-
 Serratia fonticola UTAD54 (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Serratia fonticola (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefepime + H2O
-
 Serratia fonticola UTAD54 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefoxitin + H2O
-
 Serratia fonticola (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Serratia fonticola UTAD54 (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Serratia fonticola (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Serratia fonticola (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
moxalactam + H2O
-
 Serratia fonticola (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
 ?
moxalactam + H2O
-
 Serratia fonticola UTAD54 (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Serratia fonticola (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
Sfh-I
-
 Serratia fonticola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 moxalactam pH 7.0, 25°C Serratia fonticola
0.032
-
 cefoxitin pH 7.0, 25°C Serratia fonticola
0.06
-
 nitrocefin pH 7.0, 25°C Serratia fonticola
0.64
-
 cefepime pH 7.0, 25°C Serratia fonticola
9.15
-
 carbenicillin pH 7.0, 25°C Serratia fonticola
9.5
-
 ampicillin pH 7.0, 25°C Serratia fonticola
51
-
 Imipenem pH 7.0, 25°C Serratia fonticola
109
-
 meropenem pH 7.0, 25°C Serratia fonticola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.18
-
 moxalactam pH 7.0, 25°C Serratia fonticola
0.32
-
 cefoxitin pH 7.0, 25°C Serratia fonticola
0.57
-
 nitrocefin pH 7.0, 25°C Serratia fonticola
3.16
-
 cefepime pH 7.0, 25°C Serratia fonticola
7.18
-
 carbenicillin pH 7.0, 25°C Serratia fonticola
14.1
-
 ampicillin pH 7.0, 25°C Serratia fonticola
505
-
 meropenem pH 7.0, 25°C Serratia fonticola
642
-
 Imipenem pH 7.0, 25°C Serratia fonticola