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Literature summary for 3.5.2.6 extracted from

  • Borgianni, L.; Prandi, S.; Salden, L.; Santella, G.; Hanson, N.D.; Rossolini, G.M.; Docquier, J.D.
    Genetic context and biochemical characterization of the IMP-18 metallo-beta-lactamase identified in a Pseudomonas aeruginosa isolate from the United States (2011), Antimicrob. Agents Chemother., 55, 140-145.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 cephalotin pH 7.5, 30°C Pseudomonas aeruginosa
0.0008
-
 cefepime pH 7.5, 30°C Pseudomonas aeruginosa
0.00084
-
 meropenem pH 7.5, 30°C Pseudomonas aeruginosa
0.0013
-
 ceftazidime pH 7.5, 30°C Pseudomonas aeruginosa
0.0026
-
 ertapenem pH 7.5, 30°C Pseudomonas aeruginosa
0.003
-
 cefotaxime pH 7.5, 30°C Pseudomonas aeruginosa
0.007
-
 Imipenem pH 7.5, 30°C Pseudomonas aeruginosa
0.007
-
 cefuroxime pH 7.5, 30°C Pseudomonas aeruginosa
0.009
-
 nitrocefin pH 7.5, 30°C Pseudomonas aeruginosa
0.011
-
 cefoxitin pH 7.5, 30°C Pseudomonas aeruginosa
0.09
-
 benzylpenicillin pH 7.5, 30°C Pseudomonas aeruginosa
0.103
-
 ampicillin pH 7.5, 30°C Pseudomonas aeruginosa
0.18
-
 ticarcillin pH 7.5, 30°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q5U807
-
-
Pseudomonas aeruginosa PS297 Q5U807
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Pseudomonas aeruginosa PS297 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefepime + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefuroxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephalotin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ertapenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl]sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
imipenem + H2O
-
 Pseudomonas aeruginosa (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ticarcillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
IMP-18
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 ertapenem pH 7.5, 30°C Pseudomonas aeruginosa
0.05
-
 meropenem pH 7.5, 30°C Pseudomonas aeruginosa
0.35
-
 cefepime pH 7.5, 30°C Pseudomonas aeruginosa
0.4
-
 cephalotin pH 7.5, 30°C Pseudomonas aeruginosa
0.7
-
 cefotaxime pH 7.5, 30°C Pseudomonas aeruginosa
0.9
-
 cefuroxime pH 7.5, 30°C Pseudomonas aeruginosa
1
-
 ceftazidime pH 7.5, 30°C Pseudomonas aeruginosa
2
-
 cefoxitin pH 7.5, 30°C Pseudomonas aeruginosa
17
-
 Imipenem pH 7.5, 30°C Pseudomonas aeruginosa
30
-
 ticarcillin pH 7.5, 30°C Pseudomonas aeruginosa
54
-
 benzylpenicillin pH 7.5, 30°C Pseudomonas aeruginosa
55
-
 nitrocefin pH 7.5, 30°C Pseudomonas aeruginosa
65
-
 ampicillin pH 7.5, 30°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4
-
 meropenem pH 7.5, 30°C Pseudomonas aeruginosa
6
-
 ertapenem pH 7.5, 30°C Pseudomonas aeruginosa
130
-
 cefuroxime pH 7.5, 30°C Pseudomonas aeruginosa
150
-
 ticarcillin pH 7.5, 30°C Pseudomonas aeruginosa
180
-
 cefoxitin pH 7.5, 30°C Pseudomonas aeruginosa
230
-
 cefotaxime pH 7.5, 30°C Pseudomonas aeruginosa
440
-
 cefepime pH 7.5, 30°C Pseudomonas aeruginosa
620
-
 benzylpenicillin pH 7.5, 30°C Pseudomonas aeruginosa
630
-
 ampicillin pH 7.5, 30°C Pseudomonas aeruginosa
770
-
 ceftazidime pH 7.5, 30°C Pseudomonas aeruginosa
2400
-
 Imipenem pH 7.5, 30°C Pseudomonas aeruginosa
4000
-
 cephalotin pH 7.5, 30°C Pseudomonas aeruginosa
6100
-
 nitrocefin pH 7.5, 30°C Pseudomonas aeruginosa