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Literature summary for 3.5.2.6 extracted from

  • Docquier, J.D.; Calderone, V.; De Luca, F.; Benvenuti, M.; Giuliani, F.; Bellucci, L.; Tafi, A.; Nordmann, P.; Botta, M.; Rossolini, G.M.; Mangani, S.
    Crystal structure of the OXA-48 beta-lactamase reveals mechanistic diversity among class D carbapenemases (2009), Chem. Biol., 16, 540-547.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop method, crystal structure of the OXA-48 carbapenemase is determined at pH 7.5 and at a resolution of 1.9 A. The enzyme shows a noncrystallographic pseudo 2-fold axis Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
meropenem pH 7.0, 30°C Klebsiella pneumoniae
0.013
-
Imipenem pH 7.0, 30°C Klebsiella pneumoniae
0.013
-
faropenem pH 7.0, 30°C Klebsiella pneumoniae
0.014
-
panipenem pH 7.0, 30°C Klebsiella pneumoniae
0.045
-
temocillin pH 7.0, 30°C Klebsiella pneumoniae
0.095
-
Oxacillin pH 7.0, 30°C Klebsiella pneumoniae
0.1
-
ertapenem pH 7.0, 30°C Klebsiella pneumoniae
0.12
-
nitrocefin pH 7.0, 30°C Klebsiella pneumoniae
0.195
-
cephalothin pH 7.0, 30°C Klebsiella pneumoniae
0.395
-
ampicillin pH 7.0, 30°C Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q6XEC0 the recombinant plasmid pVT-1 is used as a source of the blaOXA-48 gene. The blaOXA-48 ORF is subcloned into expression vector pET-9a to yield recombinant plasmid pET-OXA-48. Escherichia coli MCT236(DE3) is used as the host for overproduction of the OXA-48 enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
Klebsiella pneumoniae (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cefepime + H2O
-
Klebsiella pneumoniae (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefotaxime + H2O
-
Klebsiella pneumoniae (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefoxitin + H2O
-
Klebsiella pneumoniae (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
ceftazidime + H2O
-
Klebsiella pneumoniae (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cephalothin + H2O
-
Klebsiella pneumoniae (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
ertapenem + H2O
-
Klebsiella pneumoniae (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl]sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
faropenem + H2O
-
Klebsiella pneumoniae (2R)-2-[(1S,2R)-1-carboxy-2-hydroxypropyl]-5-[(2R)-tetrahydrofuran-2-yl]-2,3-dihydro-1,3-thiazole-4-carboxylic acid
-
?
imipenem + H2O
-
Klebsiella pneumoniae (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
meropenem + H2O
-
Klebsiella pneumoniae (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
nitrocefin + H2O
-
Klebsiella pneumoniae (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
oxacillin + H2O
-
Klebsiella pneumoniae (2R,4S)-2-[(R)-carboxy[[(5-methyl-3-phenyl-1,2-oxazol-4-yl)carbonyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
panipenem + H2O
-
Klebsiella pneumoniae (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S)-1-ethanimidoylpyrrolidin-3-yl]sulfanyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
temocillin + H2O
-
Klebsiella pneumoniae (2R,4S)-2-[(S)-carboxy[[carboxy(thiophen-3-yl)acetyl]amino]methoxymethyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Subunits

Subunits Comment Organism
dimer
-
Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
OXA-48 carbapenemase
-
Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
faropenem pH 7.0, 30°C Klebsiella pneumoniae
0.07
-
meropenem pH 7.0, 30°C Klebsiella pneumoniae
0.13
-
ertapenem pH 7.0, 30°C Klebsiella pneumoniae
0.3
-
temocillin pH 7.0, 30°C Klebsiella pneumoniae
1.4
-
panipenem pH 7.0, 30°C Klebsiella pneumoniae
4.8
-
Imipenem pH 7.0, 30°C Klebsiella pneumoniae
44
-
cephalothin pH 7.0, 30°C Klebsiella pneumoniae
130
-
Oxacillin pH 7.0, 30°C Klebsiella pneumoniae
940
-
nitrocefin pH 7.0, 30°C Klebsiella pneumoniae
955
-
ampicillin pH 7.0, 30°C Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.26
-
cefoxitin pH 7.0, 30°C Klebsiella pneumoniae
1.1
-
cefepime pH 7.0, 30°C Klebsiella pneumoniae
1.3
-
ertapenem pH 7.0, 30°C Klebsiella pneumoniae
2.9
-
faropenem pH 7.0, 30°C Klebsiella pneumoniae
6.2
-
meropenem pH 7.0, 30°C Klebsiella pneumoniae
6.6
-
temocillin pH 7.0, 30°C Klebsiella pneumoniae
10
-
cefotaxime pH 7.0, 30°C Klebsiella pneumoniae
100
-
panipenem pH 7.0, 30°C Klebsiella pneumoniae
230
-
cephalothin pH 7.0, 30°C Klebsiella pneumoniae
370
-
Imipenem pH 7.0, 30°C Klebsiella pneumoniae
1400
-
Oxacillin pH 7.0, 30°C Klebsiella pneumoniae
2400
-
ampicillin pH 7.0, 30°C Klebsiella pneumoniae
7700
-
nitrocefin pH 7.0, 30°C Klebsiella pneumoniae