Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Aeromonas veronii bv. sobria |
Protein Variants | Comment | Organism |
---|---|---|
E152C | ImiS mutant (ImiS[E152C]) is generated by mutating the imiS gene in pET26bimiS. Mutant exhibits very similar kinetic behavior to wild type | Aeromonas veronii bv. sobria |
spin-labeled ImiS | ImiS* | Aeromonas veronii bv. sobria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.104 | - |
Imipenem | spin-labeled enzyme, pH 7.0, 25°C | Aeromonas veronii bv. sobria | |
0.106 | - |
Imipenem | E152C mutant, pH 7.0, 25°C | Aeromonas veronii bv. sobria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | metalloenzyme | Aeromonas veronii bv. sobria | |
Zn2+ | metalloenzyme, E152C mutant contains 0.80 equivalents of Zn, which is similar to the metal content of wild type enzyme | Aeromonas veronii bv. sobria |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aeromonas veronii bv. sobria | readily hydrolyzes carbapenems | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas veronii bv. sobria | O31272 | - |
- |
Purification (Comment) | Organism |
---|---|
E152C mutant is purified | Aeromonas veronii bv. sobria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
imipenem + H2O | - |
Aeromonas veronii bv. sobria | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
additional information | readily hydrolyzes carbapenems | Aeromonas veronii bv. sobria | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ImiS | - |
Aeromonas veronii bv. sobria |
MbetaL | - |
Aeromonas veronii bv. sobria |
metallo-beta-lactamase | - |
Aeromonas veronii bv. sobria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aeromonas veronii bv. sobria |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
292 | - |
Imipenem | E152C mutant, pH 7.0, 25°C | Aeromonas veronii bv. sobria | |
295 | - |
Imipenem | spin-labeled enzyme, pH 7.0, 25°C | Aeromonas veronii bv. sobria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aeromonas veronii bv. sobria |