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Literature summary for 3.5.2.6 extracted from

  • Nagano, Y.; Nagano, N.; Wachino, J.; Ishikawa, K.; Arakawa, Y.
    Novel chimeric beta -lactamase CTX-M-64, a hybrid of CTX-M-15-like and CTX-M-14 beta -lactamases, found in a Shigella sonnei strain resistant to various oxyimino-cephalosporins, including ceftazidime (2009), Antimicrob. Agents Chemother., 53, 69-74.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Shigella sonnei

Inhibitors

Inhibitors Comment Organism Structure
ampicillin
-
Shigella sonnei
cefepime
-
Shigella sonnei
cefotaxime
-
Shigella sonnei
ceftazidime
-
Shigella sonnei
cephalothin
-
Shigella sonnei
clavulanic acid reduces the hydrolysis rate of 100 microM nitrocefin by 50% when the enzyme is preincubated with various concentrations of the inhibitor for 5 min at 37°C before the addition of the substrate Shigella sonnei
nitrocefin
-
Shigella sonnei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0148
-
nitrocefin 37°C, pH 7.0 Shigella sonnei
0.0195
-
ampicillin 37°C, pH 7.0 Shigella sonnei
0.0379
-
cephalothin 37°C, pH 7.0 Shigella sonnei
0.103
-
cefotaxime 37°C, pH 7.0 Shigella sonnei
0.505
-
cefepime 37°C, pH 7.0 Shigella sonnei

Organism

Organism UniProt Comment Textmining
Shigella sonnei A5LH21
-
-
Shigella sonnei UIH-1 A5LH21
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Shigella sonnei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
Shigella sonnei (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
ampicillin + H2O
-
Shigella sonnei UIH-1 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cefepime + H2O
-
Shigella sonnei (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefepime + H2O
-
Shigella sonnei UIH-1 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefotaxime + H2O
-
Shigella sonnei (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefotaxime + H2O
-
Shigella sonnei UIH-1 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
ceftazidime + H2O
-
Shigella sonnei (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
ceftazidime + H2O
-
Shigella sonnei UIH-1 (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cephalothin + H2O
-
Shigella sonnei (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cephalothin + H2O
-
Shigella sonnei UIH-1 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
nitrocefin + H2O
-
Shigella sonnei (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?

Synonyms

Synonyms Comment Organism
beta-lactamase
-
Shigella sonnei
CTX-M-64 a hybrid of CTX-M-15-like and CTX-M-14 beta-lactamases Shigella sonnei
CTX-M-64 beta-lactamase
-
Shigella sonnei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Shigella sonnei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
36.9
-
ampicillin 37°C, pH 7.0 Shigella sonnei
67.7
-
cefepime 37°C, pH 7.0 Shigella sonnei
185
-
cephalothin 37°C, pH 7.0 Shigella sonnei
197
-
cefotaxime 37°C, pH 7.0 Shigella sonnei
252
-
nitrocefin 37°C, pH 7.0 Shigella sonnei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Shigella sonnei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0148
-
nitrocefin 37°C, pH 7.0 Shigella sonnei
0.0195
-
ampicillin 37°C, pH 7.0 Shigella sonnei
0.0379
-
cephalothin 37°C, pH 7.0 Shigella sonnei
0.103
-
cefotaxime 37°C, pH 7.0 Shigella sonnei
0.505
-
cefepime 37°C, pH 7.0 Shigella sonnei

pI Value

Organism Comment pI Value Maximum pI Value
Shigella sonnei more than
-
8.7