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Literature summary for 3.5.2.6 extracted from

  • Mammeri, H.; Galleni, M.; Nordmann, P.
    Role of the Ser-287-Asn replacement in the hydrolysis spectrum extension of AmpC beta -lactamases in Escherichia coli (2009), Antimicrob. Agents Chemother., 53, 323-326.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Protein Variants

Protein Variants Comment Organism
S287N the AmpC variants AmpC-A and AmpC-B2 harboring the S287N substitution are obtained by mutagenesis from cephalosporinases representative of the phylogenetic groups A and B2 of Escherichia coli. Their biochemical characterization reveals that the S287N replacement leads to an important increase in the catalytic efficiency toward extended-spectrum cephalosporins in the AmpC beta-lactamase of group A only Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information for substrate cefepime, wild-type AmpC-B2 and AmpC-A, Km-value is above 1000 mM Escherichia coli
0.1
-
 cefotaxime mutant AmpC-A-S287N Escherichia coli
0.2
-
 Imipenem mutant AmpC-A-S287N Escherichia coli
0.4
-
 ceftazidime mutant AmpC-A-S287N Escherichia coli
0.7
-
 cefoxitin mutant AmpC-A-S287N Escherichia coli
0.8
-
 cefoxitin mutant AmpC-B2-S287N Escherichia coli
1
-
 cefotaxime mutant AmpC-B2-S287N Escherichia coli
1
-
 ceftazidime mutant AmpC-B2-S287N Escherichia coli
1
-
 cefoxitin wild-type AmpC-A Escherichia coli
1.5
-
 cefoxitin wild-type AmpC-B2 Escherichia coli
2
-
 Imipenem mutant AmpC-B2-S287N Escherichia coli
5.5
-
 cephalothin mutant AmpC-A-S287N Escherichia coli
10
-
 Imipenem wild-type AmpC-A Escherichia coli
25
-
 cefotaxime wild-type AmpC-A Escherichia coli
30
-
 cephalothin wild-type AmpC-A Escherichia coli
32
-
 Imipenem wild-type AmpC-B2 Escherichia coli
37
-
 cefepime mutant AmpC-B2-S287N Escherichia coli
45
-
 cephalothin mutant AmpC-B2-S287N Escherichia coli
45
-
 cefotaxime wild-type AmpC-B2 Escherichia coli
60
-
 cephaloridine mutant AmpC-A-S287N Escherichia coli
70
-
 ceftazidime wild-type AmpC-A Escherichia coli
75
-
 cefepime mutant AmpC-A-S287N Escherichia coli
85
-
 cephalothin wild-type AmpC-B2 Escherichia coli
130
-
 cephaloridine mutant AmpC-B2-S287N Escherichia coli
270
-
 ceftazidime wild-type AmpC-B2 Escherichia coli
650
-
 cephaloridine wild-type AmpC-A Escherichia coli
950
-
 cephaloridine wild-type AmpC-B2 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00811
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cefepime + H2O
-
 Escherichia coli (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Escherichia coli (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephaloridine + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephalothin + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Escherichia coli (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
AmpC beta-lactamase
-
 Escherichia coli
AmpC-A
-
 Escherichia coli
AmpC-B2
-
 Escherichia coli
beta-lactamase
-
 Escherichia coli
Cephalosporinase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 ceftazidime mutant AmpC-B2-S287N Escherichia coli
0.045
-
 cefotaxime mutant AmpC-B2-S287N Escherichia coli
0.06
-
 Imipenem mutant AmpC-A-S287N Escherichia coli
0.06
-
 Imipenem mutant AmpC-B2-S287N Escherichia coli
0.08
-
 cefotaxime mutant AmpC-A-S287N Escherichia coli
0.08
-
 cefoxitin mutant AmpC-B2-S287N Escherichia coli
0.09
-
 Imipenem wild-type AmpC-B2 Escherichia coli
0.1
-
 cefoxitin mutant AmpC-A-S287N Escherichia coli
0.1
-
 Imipenem wild-type AmpC-A Escherichia coli
0.1
-
 cefotaxime wild-type AmpC-B2 Escherichia coli
0.2
-
 ceftazidime mutant AmpC-A-S287N Escherichia coli
0.2
-
 cefotaxime wild-type AmpC-A Escherichia coli
0.25
-
 cefepime mutant AmpC-B2-S287N Escherichia coli
0.3
-
 cefoxitin wild-type AmpC-A Escherichia coli
0.3
-
 ceftazidime wild-type AmpC-B2 Escherichia coli
0.4
-
 cefoxitin wild-type AmpC-B2 Escherichia coli
0.5
-
 ceftazidime wild-type AmpC-A Escherichia coli
1.35
-
 cefepime mutant AmpC-A-S287N Escherichia coli
10
-
 cephalothin mutant AmpC-B2-S287N Escherichia coli
50
-
 cephaloridine mutant AmpC-A-S287N Escherichia coli
65
-
 cephalothin mutant AmpC-A-S287N Escherichia coli
112
-
 cephaloridine mutant AmpC-B2-S287N Escherichia coli
230
-
 cephaloridine wild-type AmpC-A Escherichia coli
240
-
 cephaloridine wild-type AmpC-B2 Escherichia coli
500
-
 cephalothin wild-type AmpC-A Escherichia coli
540
-
 cephalothin wild-type AmpC-B2 Escherichia coli