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Literature summary for 3.5.2.6 extracted from
- Kinetic characterization of VIM-7, a divergent member of the VIM metallo-beta -lactamase family (2008), Antimicrob. Agents Chemother., 52, 2905-2908.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y218F | substitution may be responsible for the reduced catalytic efficiency against certain cephalosporins, including ceftazidime and cefepime | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| clavulanic acid | - |
Pseudomonas aeruginosa |  |
| additional information | metallo-beta-lactamases are not inhibited by clinical beta-lactamase inhibitors | Pseudomonas aeruginosa | |
| Sulbactam | - |
Pseudomonas aeruginosa | |
| tazobactam | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
ampicillin | - |
Pseudomonas aeruginosa | |
| 0.017 | - |
benzylpenicillin | - |
Pseudomonas aeruginosa | |
| 0.022 | - |
cefotaxime | - |
Pseudomonas aeruginosa | |
| 0.026 | - |
piperacillin | - |
Pseudomonas aeruginosa | |
| 0.027 | - |
Imipenem | - |
Pseudomonas aeruginosa | |
| 0.028 | - |
ertapenem | - |
Pseudomonas aeruginosa | |
| 0.029 | - |
cefuroxime | - |
Pseudomonas aeruginosa | |
| 0.038 | - |
meropenem | - |
Pseudomonas aeruginosa | |
| 0.045 | - |
cephalothin | - |
Pseudomonas aeruginosa | |
| 0.058 | - |
nitrocefin | - |
Pseudomonas aeruginosa | |
| 0.066 | - |
azlocillin | - |
Pseudomonas aeruginosa | |
| 0.068 | - |
cefoxitin | - |
Pseudomonas aeruginosa | |
| 0.075 | - |
moxalactam | - |
Pseudomonas aeruginosa | |
| 0.084 | - |
carbenicillin | - |
Pseudomonas aeruginosa | |
| 0.12 | - |
ceftazidime | - |
Pseudomonas aeruginosa | |
| 0.25 | - |
cephaloridine | - |
Pseudomonas aeruginosa | |
| 0.58 | - |
cefepime | - |
Pseudomonas aeruginosa | |
| 0.86 | - |
Cloxacillin | - |
Pseudomonas aeruginosa | |
| 2.7 | - |
aztreonam | - |
Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q840P9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange and gel filtration | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ampicillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| azlocillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-carboxy({(2R)-2-[(2-oxoimidazolidine-1-carbonyl)amino]-2-phenylacetyl}amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| aztreonam + H2O | - |
Pseudomonas aeruginosa | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
| benzylpenicillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| carbenicillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cefepime + H2O | - |
Pseudomonas aeruginosa | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| cefotaxime + H2O | - |
Pseudomonas aeruginosa | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cefoxitin + H2O | - |
Pseudomonas aeruginosa | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| ceftazidime + H2O | - |
Pseudomonas aeruginosa | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| cefuroxime + H2O | - |
Pseudomonas aeruginosa | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephaloridine + H2O | - |
Pseudomonas aeruginosa | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| cephalothin + H2O | - |
Pseudomonas aeruginosa | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cloxacillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ertapenem + H2O | - |
Pseudomonas aeruginosa | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl}sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| imipenem + H2O | - |
Pseudomonas aeruginosa | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| meropenem + H2O | - |
Pseudomonas aeruginosa | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| moxalactam + H2O | - |
Pseudomonas aeruginosa | (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid | - |
? | |
| nitrocefin + H2O | - |
Pseudomonas aeruginosa | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| piperacillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cephalosporinase | - |
Pseudomonas aeruginosa |
| MBL | - |
Pseudomonas aeruginosa |
| metallo-beta-lactamase | - |
Pseudomonas aeruginosa |
| VIM-7 | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
ceftazidime | - |
Pseudomonas aeruginosa | |
| 5.3 | - |
cefepime | - |
Pseudomonas aeruginosa | |
| 8 | - |
ertapenem | - |
Pseudomonas aeruginosa | |
| 10 | - |
cefoxitin | - |
Pseudomonas aeruginosa | |
| 16 | - |
cefuroxime | - |
Pseudomonas aeruginosa | |
| 42 | - |
meropenem | - |
Pseudomonas aeruginosa | |
| 56 | - |
cefotaxime | - |
Pseudomonas aeruginosa | |
| 78 | - |
azlocillin | - |
Pseudomonas aeruginosa | |
| 100 | - |
Imipenem | - |
Pseudomonas aeruginosa | |
| 140 | - |
piperacillin | - |
Pseudomonas aeruginosa | |
| 180 | - |
cephalothin | - |
Pseudomonas aeruginosa | |
| 180 | - |
cephaloridine | - |
Pseudomonas aeruginosa | |
| 190 | - |
ampicillin | - |
Pseudomonas aeruginosa | |
| 230 | - |
moxalactam | - |
Pseudomonas aeruginosa | |
| 430 | - |
benzylpenicillin | - |
Pseudomonas aeruginosa | |
| 1200 | - |
carbenicillin | - |
Pseudomonas aeruginosa | |
| 1500 | - |
nitrocefin | - |
Pseudomonas aeruginosa | |
| 2500 | - |
Cloxacillin | - |
Pseudomonas aeruginosa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.74 | - |
Sulbactam | - |
Pseudomonas aeruginosa | |
| 0.94 | - |
clavulanic acid | - |
Pseudomonas aeruginosa | |
| 3.5 | - |
tazobactam | - |
Pseudomonas aeruginosa | |
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