Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 67% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
Cd2+ | 24% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
Cu2+ | 42% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
EDTA | complete inhibition at 0.000001 mM | Neisseria gonorrhoeae | |
Fe2+ | 50% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
Fe3+ | 24% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
Hg2+ | complete inhibition at 0.000001 mM | Neisseria gonorrhoeae | |
Mg2+ | 97% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
Mn2+ | 49% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
Ni2+ | 28% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
p-chloromercuribenzoate | 2% residual activity at 0.01 mM | Neisseria gonorrhoeae | |
Zn2+ | 6% residual activity at 0.01 mM | Neisseria gonorrhoeae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Neisseria gonorrhoeae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephadex G75 gel filtration, and DEAE-cellulose column chromatography | Neisseria gonorrhoeae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
crude extract | Neisseria gonorrhoeae |
13.67 | - |
after 37.97fold purification | Neisseria gonorrhoeae |
Storage Stability | Organism |
---|---|
-30°C, 30 days, 79% loss of activity | Neisseria gonorrhoeae |
-70°C, 2 months, no loss of activity | Neisseria gonorrhoeae |
-70°C, 5 months, 22% loss of activity | Neisseria gonorrhoeae |
25°C, 25 days, complete loss of activity | Neisseria gonorrhoeae |
4°C, 5 days, 50% loss of activity | Neisseria gonorrhoeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephaloridine + H2O | - |
Neisseria gonorrhoeae | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Neisseria gonorrhoeae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Neisseria gonorrhoeae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 55 | residual activity of 82% after 25 min at 37°C and complete loss of activity after 1 min at 60°C or 5 min at 55°C | Neisseria gonorrhoeae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.2 | in 50 mM phosphate buffer | Neisseria gonorrhoeae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8 | at pH values higher than 7.2 a decrease of the enzyme activity is observed, having lost 50% at pH 8.0 | Neisseria gonorrhoeae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Neisseria gonorrhoeae | isoelectric focusing | - |
5.4 |