Literature summary for 3.5.2.6 extracted from

Fonseca, F.; Sarmento, A.C.; Henriques, I.; Samyn, B.; van Beeumen, J.; Domingues,P.; Domingues, M.R.; Saavedra, M.J.; and Correia, A.
Biochemical characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase (2007), Antimicrob. Agents Chemother., 51, 4512-4514.

Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)(pMF13) cells	Serratia fonticola

Inhibitors

Inhibitors	Comment	Organism	Structure
clavulanic acid	-	Serratia fonticola
Sulbactam	-	Serratia fonticola
tazobactam	-	Serratia fonticola

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.026	-	meropenem	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.038	-	piperacillin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.052	-	ceftazidime	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.077	-	cefoxitin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.082	-	Imipenem	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.089	-	cefotaxime	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.129	-	cephalothin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.176	-	ampicillin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
0.484	-	aztreonam	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30500	-	SDS-PAGE	Serratia fonticola
30770	-	mature protein, electrospray ionization-mass spectrometry	Serratia fonticola
31080	-	electrospray ionization-mass spectrometry	Serratia fonticola

Organism

Organism	UniProt	Comment	Textmining
Serratia fonticola	-	-	-
Serratia fonticola UTAD54	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
S-Sepharose column chromatography	Serratia fonticola

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a penicillin + H2O	-	Serratia fonticola	a penicilloic acid	-	?
a penicillin + H2O	-	Serratia fonticola UTAD54	a penicilloic acid	-	?
ampicillin + H2O	-	Serratia fonticola	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ampicillin + H2O	-	Serratia fonticola UTAD54	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
aztreonam + H2O	-	Serratia fonticola	[(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate	-	?
aztreonam + H2O	-	Serratia fonticola UTAD54	[(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate	-	?
carbapenem + H2O	-	Serratia fonticola	[(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid	-	?
cefotaxime + H2O	-	Serratia fonticola	(2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefotaxime + H2O	-	Serratia fonticola UTAD54	(2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefoxitin + H2O	-	Serratia fonticola	(2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
ceftazidime + H2O	-	Serratia fonticola	(2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
cephalosporin + H2O	-	Serratia fonticola	?	-	?
cephalothin + H2O	-	Serratia fonticola	(2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalothin + H2O	-	Serratia fonticola UTAD54	(2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
imipenem + H2O	-	Serratia fonticola	(5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
meropenem + H2O	-	Serratia fonticola	(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
piperacillin + H2O	-	Serratia fonticola	(2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Synonyms

Synonyms	Comment	Organism
SFC-1	-	Serratia fonticola

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1	-	ceftazidime	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
4.2	-	cefoxitin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
6.5	-	meropenem	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
8.3	-	cefotaxime	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
44.7	-	piperacillin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
54	-	Imipenem	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
155	-	ampicillin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
162	-	aztreonam	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola
280	-	cephalothin	at 25°C in 50 mM sodium phosphate (pH 7.0)	Serratia fonticola

pI Value

Organism	Comment	pI Value Maximum	pI Value
Serratia fonticola	purified SFC-1 migrates as a single band, but isoelectric focusing reveals two forms of purified SFC-1, with pI values of 7.6 and 8.2	-	7.6
Serratia fonticola	purified SFC-1 migrates as a single band, but isoelectric focusing reveals two forms of purified SFC-1, with pI values of 7.6 and 8.2	-	8.2

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)