Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Alkalihalobacillus clausii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clavulanic acid | - |
Alkalihalobacillus clausii | |
Sulbactam | - |
Alkalihalobacillus clausii | |
tazobactam | - |
Alkalihalobacillus clausii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
cefoxitin | Km less than 0.00001 mM at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.006 | - |
ticarcillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.012 | - |
benzylpenicillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.015 | - |
cephalothin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.021 | - |
piperacillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.022 | - |
amoxicillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.035 | - |
Imipenem | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.045 | - |
cephaloridine | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.105 | - |
cefuroxime | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.136 | - |
cefpirome | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.79 | - |
cefotaxime | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.95 | - |
ceftriaxone | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
1 | - |
ceftazidime | Km above 1.0 mM at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
1 | - |
cefepime | Km above 1.0 mM at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE | Alkalihalobacillus clausii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus clausii | A8RR46 | - |
- |
Alkalihalobacillus clausii NR | A8RR46 | - |
- |
Purification (Comment) | Organism |
---|---|
S-Sepharose column chromatography and Q-Sepharose column chromatography | Alkalihalobacillus clausii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
The specific activity of purified BCL-1, measured with 0.1 mM of cephalothin as the substrate, was 35 units/mg of protein with a 26fold purification factor | Alkalihalobacillus clausii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amoxicillin + H2O | - |
Alkalihalobacillus clausii | (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
aztreonam + H2O | - |
Alkalihalobacillus clausii | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
aztreonam + H2O | - |
Alkalihalobacillus clausii NR | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
benzylpenicillin + H2O | - |
Alkalihalobacillus clausii | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Alkalihalobacillus clausii NR | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefepime + H2O | - |
Alkalihalobacillus clausii | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefotaxime + H2O | - |
Alkalihalobacillus clausii | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | - |
Alkalihalobacillus clausii | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefpirome + H2O | - |
Alkalihalobacillus clausii | (2S)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-5-[(6,7-dihydro-5H-cyclopenta[b]pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
ceftazidime + H2O | - |
Alkalihalobacillus clausii | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
ceftriaxone + H2O | - |
Alkalihalobacillus clausii | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[[(6-hydroxy-2-methyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefuroxime + H2O | - |
Alkalihalobacillus clausii | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefuroxime + H2O | - |
Alkalihalobacillus clausii NR | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | - |
Alkalihalobacillus clausii | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephaloridine + H2O | - |
Alkalihalobacillus clausii NR | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
Alkalihalobacillus clausii | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalothin + H2O | - |
Alkalihalobacillus clausii NR | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Alkalihalobacillus clausii | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
moxalactam + H2O | - |
Alkalihalobacillus clausii | (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Alkalihalobacillus clausii | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ticarcillin + H2O | - |
Alkalihalobacillus clausii | (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCL-1 | - |
Alkalihalobacillus clausii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
cefoxitin | kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.01 | - |
moxalactam | kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.06 | - |
Imipenem | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
0.3 | - |
aztreonam | kcat less than 0.3/sec at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
3.3 | - |
ceftazidime | kcat above than 3.3/sec at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
5 | - |
ticarcillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
15 | - |
cefuroxime | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
22 | - |
cephalothin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
22 | - |
cefotaxime | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
30 | - |
ceftriaxone | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
38 | - |
piperacillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
40 | - |
cefepime | kcat above 40/sec at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
85 | - |
amoxicillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
95 | - |
cefpirome | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
97 | - |
benzylpenicillin | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii | |
115 | - |
cephaloridine | at 30°C in 100 mM sodium phosphate (pH 7) | Alkalihalobacillus clausii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Alkalihalobacillus clausii | isoelectric focusing | - |
5.5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000004 | - |
using cephalothin as a substrate, at 30°C | Alkalihalobacillus clausii | tazobactam | |
0.000075 | - |
using cephalothin as a substrate, at 30°C | Alkalihalobacillus clausii | Sulbactam | |
0.000085 | - |
using cephalothin as a substrate, at 30°C | Alkalihalobacillus clausii | clavulanic acid |