Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Shewanella livingstonensis |
expression in Escherichia coli | Shewanella frigidimarina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipicolinic acid | IC50: above 0.5 mM | Shewanella frigidimarina | |
dipicolinic acid | IC50: 0.05 mM | Shewanella livingstonensis | |
EDTA | IC50: 75 mM | Shewanella frigidimarina | |
EDTA | IC50: 0.05 mM | Shewanella livingstonensis | |
additional information | no inhibition by clavulanate | Shewanella livingstonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
cefuroxime | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.009 | - |
ceftazidime | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.012 | - |
cefalothin | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.043 | - |
meropenem | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.08 | - |
cefalothin | 30°C, pH 7.0 | Shewanella frigidimarina | |
0.115 | - |
ticarcillin | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.17 | - |
piperacillin | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.19 | - |
amoxicillin | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.26 | - |
benzylpenicillin | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.27 | - |
cefaloridine | 30°C, pH 7.0 | Shewanella livingstonensis | |
0.8 | - |
cefaloridine | 30°C, pH 7.0 | Shewanella frigidimarina | |
1.8 | - |
Imipenem | 30°C, pH 7.0 | Shewanella frigidimarina | |
2.4 | - |
moxalactam | 30°C, pH 7.0 | Shewanella frigidimarina | |
2.5 | - |
Imipenem | 30°C, pH 7.0 | Shewanella livingstonensis | |
2.6 | - |
amoxicillin | 30°C, pH 7.0 | Shewanella frigidimarina | |
6 | - |
aztreonam | 30°C, pH 7.0 | Shewanella frigidimarina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella frigidimarina | Q53BU0 | - |
- |
Shewanella livingstonensis | Q53BU1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Shewanella livingstonensis |
- |
Shewanella frigidimarina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amoxicillin + H2O | - |
Shewanella livingstonensis | (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
amoxicillin + H2O | - |
Shewanella frigidimarina | (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
aztreonam + H2O | - |
Shewanella frigidimarina | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
benzylpenicillin + H2O | 670% of the activity with amoxicillin | Shewanella livingstonensis | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefaloridine + H2O | 25% of the activity with amoxicillin | Shewanella livingstonensis | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefaloridine + H2O | 3% of the activity with amoxicillin | Shewanella frigidimarina | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefalotin + H2O | 3% of the activity with amoxicillin | Shewanella frigidimarina | (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefalotin + H2O | 8% of the activity with amoxicillin | Shewanella livingstonensis | (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | 5% of the activity with amoxicillin | Shewanella livingstonensis | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefuroxime + H2O | 2% of the activity with amoxicillin | Shewanella livingstonensis | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | 5% of the activity with amoxicillin | Shewanella frigidimarina | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
imipenem + H2O | as active as amoxicillin | Shewanella livingstonensis | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
meropenem + H2O | 230% of the activity with amoxicillin | Shewanella livingstonensis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
moxalactam + H2O | 230% of the activity with amoxicillin | Shewanella frigidimarina | (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid | - |
? | |
piperacillin + H2O | 160% of the activity with amoxicillin | Shewanella livingstonensis | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ticarcillin + H2O | 130% of the activity with amoxicillin | Shewanella livingstonensis | (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbapenemase | - |
Shewanella livingstonensis |
carbapenemase | - |
Shewanella frigidimarina |
class B beta-lactamase SFB-1 | - |
Shewanella frigidimarina |
class B beta-lactamase SLB-1 | - |
Shewanella livingstonensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.05 | - |
IC50: 0.05 mM | Shewanella livingstonensis | EDTA | |
0.05 | - |
IC50: 0.05 mM | Shewanella livingstonensis | dipicolinic acid | |
0.5 | - |
IC50: above 0.5 mM | Shewanella frigidimarina | dipicolinic acid | |
75 | - |
IC50: 75 mM | Shewanella frigidimarina | EDTA |