Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.2.6 extracted from

  • Poirel, L.; Heritier, C.; Nordmann, P.
    Genetic and biochemical characterization of the chromosome-encoded class B beta-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1) (2005), J. Antimicrob. Chemother., 55, 680-685.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Shewanella livingstonensis
expression in Escherichia coli Shewanella frigidimarina

Inhibitors

Inhibitors Comment Organism Structure
dipicolinic acid IC50: above 0.5 mM Shewanella frigidimarina
dipicolinic acid IC50: 0.05 mM Shewanella livingstonensis
EDTA IC50: 75 mM Shewanella frigidimarina
EDTA IC50: 0.05 mM Shewanella livingstonensis
additional information no inhibition by clavulanate Shewanella livingstonensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
cefuroxime 30°C, pH 7.0 Shewanella livingstonensis
0.009
-
ceftazidime 30°C, pH 7.0 Shewanella livingstonensis
0.012
-
cefalothin 30°C, pH 7.0 Shewanella livingstonensis
0.043
-
meropenem 30°C, pH 7.0 Shewanella livingstonensis
0.08
-
cefalothin 30°C, pH 7.0 Shewanella frigidimarina
0.115
-
ticarcillin 30°C, pH 7.0 Shewanella livingstonensis
0.17
-
piperacillin 30°C, pH 7.0 Shewanella livingstonensis
0.19
-
amoxicillin 30°C, pH 7.0 Shewanella livingstonensis
0.26
-
benzylpenicillin 30°C, pH 7.0 Shewanella livingstonensis
0.27
-
cefaloridine 30°C, pH 7.0 Shewanella livingstonensis
0.8
-
cefaloridine 30°C, pH 7.0 Shewanella frigidimarina
1.8
-
Imipenem 30°C, pH 7.0 Shewanella frigidimarina
2.4
-
moxalactam 30°C, pH 7.0 Shewanella frigidimarina
2.5
-
Imipenem 30°C, pH 7.0 Shewanella livingstonensis
2.6
-
amoxicillin 30°C, pH 7.0 Shewanella frigidimarina
6
-
aztreonam 30°C, pH 7.0 Shewanella frigidimarina

Organism

Organism UniProt Comment Textmining
Shewanella frigidimarina Q53BU0
-
-
Shewanella livingstonensis Q53BU1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Shewanella livingstonensis
-
Shewanella frigidimarina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amoxicillin + H2O
-
Shewanella livingstonensis (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
amoxicillin + H2O
-
Shewanella frigidimarina (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
aztreonam + H2O
-
Shewanella frigidimarina [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
?
benzylpenicillin + H2O 670% of the activity with amoxicillin Shewanella livingstonensis (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cefaloridine + H2O 25% of the activity with amoxicillin Shewanella livingstonensis (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefaloridine + H2O 3% of the activity with amoxicillin Shewanella frigidimarina (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefalotin + H2O 3% of the activity with amoxicillin Shewanella frigidimarina (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefalotin + H2O 8% of the activity with amoxicillin Shewanella livingstonensis (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
ceftazidime + H2O 5% of the activity with amoxicillin Shewanella livingstonensis (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefuroxime + H2O 2% of the activity with amoxicillin Shewanella livingstonensis (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
imipenem + H2O 5% of the activity with amoxicillin Shewanella frigidimarina (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
imipenem + H2O as active as amoxicillin Shewanella livingstonensis (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
meropenem + H2O 230% of the activity with amoxicillin Shewanella livingstonensis (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
moxalactam + H2O 230% of the activity with amoxicillin Shewanella frigidimarina (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
?
piperacillin + H2O 160% of the activity with amoxicillin Shewanella livingstonensis (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
ticarcillin + H2O 130% of the activity with amoxicillin Shewanella livingstonensis (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Synonyms

Synonyms Comment Organism
carbapenemase
-
Shewanella livingstonensis
carbapenemase
-
Shewanella frigidimarina
class B beta-lactamase SFB-1
-
Shewanella frigidimarina
class B beta-lactamase SLB-1
-
Shewanella livingstonensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.05
-
IC50: 0.05 mM Shewanella livingstonensis EDTA
0.05
-
IC50: 0.05 mM Shewanella livingstonensis dipicolinic acid
0.5
-
IC50: above 0.5 mM Shewanella frigidimarina dipicolinic acid
75
-
IC50: 75 mM Shewanella frigidimarina EDTA