Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
plasmid pET28b is transformed into Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with 0.1 M Tris-HCl, pH 8.0, 2.0 M NH4H2PO4 | Mycobacterium tuberculosis |
hanging-drop vapour-diffusion method, 1.2 A resolution | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the amino acid substitutions N132G, R164A, R244A, and R276E explain the broad specificity of the enzyme, relatively low penicillinase activity, and resistance to clavulanic acid | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clavulanate | - |
Mycobacterium tuberculosis | |
clavulanic acid | poor inhibitor | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
ampicillin | - |
Mycobacterium tuberculosis | |
0.063 | - |
ampicillin | at 30°C | Mycobacterium tuberculosis | |
0.117 | - |
cephalothin | - |
Mycobacterium tuberculosis | |
0.117 | - |
cephalothin | at 30°C | Mycobacterium tuberculosis | |
0.195 | - |
cefoxitin | - |
Mycobacterium tuberculosis | |
0.195 | - |
cefoxitin | at 30°C | Mycobacterium tuberculosis | |
0.279 | - |
meropenem | - |
Mycobacterium tuberculosis | |
0.279 | - |
meropenem | at 30°C | Mycobacterium tuberculosis | |
0.593 | - |
ceftazidime | - |
Mycobacterium tuberculosis | |
0.593 | - |
ceftazidime | at 30°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30630 | - |
truncated protein, matrix-assisted laser desorption ionization | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Ni2+-loaded HiTrap chelating column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Mycobacterium tuberculosis | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | low activity | Mycobacterium tuberculosis | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | - |
Mycobacterium tuberculosis | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | - |
Mycobacterium tuberculosis | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
Mycobacterium tuberculosis | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
meropenem + H2O | - |
Mycobacterium tuberculosis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BlaC | - |
Mycobacterium tuberculosis |
class A beta-lactamase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ceftazidime | - |
Mycobacterium tuberculosis | |
0.2 | - |
ceftazidime | at 30°C | Mycobacterium tuberculosis | |
0.9 | - |
meropenem | - |
Mycobacterium tuberculosis | |
0.9 | - |
meropenem | at 30°C | Mycobacterium tuberculosis | |
1.1 | - |
cefoxitin | - |
Mycobacterium tuberculosis | |
1.1 | - |
cefoxitin | at 30°C | Mycobacterium tuberculosis | |
12.1 | - |
cephalothin | - |
Mycobacterium tuberculosis | |
12.1 | - |
cephalothin | at 30°C | Mycobacterium tuberculosis | |
18.5 | - |
ampicillin | - |
Mycobacterium tuberculosis | |
18.5 | - |
ampicillin | at 30°C | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
clavulanic acid | - |
Mycobacterium tuberculosis | |
0.0024 | - |
clavulanate | - |
Mycobacterium tuberculosis |