Literature summary for 3.5.2.6 extracted from

Girlich, D.; Naas, T.; Nordmann, P.
Biochemical characterization of the naturally occurring oxacillinase OXA-50 of Pseudomonas aeruginosa (2004), Antimicrob. Agents Chemother., 48, 2043-2048.

Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
cloned into a multicopy plasmid and expressed in Escherichia coli and Pseudomonas aeruginosa	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	Imipenem	30°C, pH 7.0	Pseudomonas aeruginosa
0.2	-	nitrocefin	30°C, pH 7.0	Pseudomonas aeruginosa
0.8	-	benzylpenicillin	30°C, pH 7.0	Pseudomonas aeruginosa
0.9	-	cephalotin	30°C, pH 7.0	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	x * 25000, SDS-PAGE	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q6PL88	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
75.5	-	-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ampicillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cefsulodin + H2O	-	Pseudomonas aeruginosa	(2R)-5-[(4-carbamoylpyridin-1-ium-1-yl)methyl]-2-[(R)-carboxy{[(2R)-2-phenyl-2-sulfoacetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
cephaloridine + H2O	-	Pseudomonas aeruginosa	(2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
cephalotin + H2O	-	Pseudomonas aeruginosa	(2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
imipenem + H2O	-	Pseudomonas aeruginosa	(5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
nitrocefin + H2O	-	Pseudomonas aeruginosa	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
oxacillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
piperacillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ticarcillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Subunits

Subunits	Comment	Organism
?	x * 25000, SDS-PAGE	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
Oxa-50	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.1	-	Imipenem	30°C, pH 7.0	Pseudomonas aeruginosa
0.2	-	cephalotin	30°C, pH 7.0	Pseudomonas aeruginosa
100	-	nitrocefin	30°C, pH 7.0	Pseudomonas aeruginosa
110	-	benzylpenicillin	30°C, pH 7.0	Pseudomonas aeruginosa

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas aeruginosa	isoelectric focusing	-	8.6

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Release 2023.1 (January 2023)