Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis | Bacillus licheniformis |
Protein Variants | Comment | Organism |
---|---|---|
K234A | no significant structural change, mutant with decreased Km 1-2 orders of magnitude and kcat 2-3 orders of magnitude | Bacillus licheniformis |
K234E | no significant structural change, mutant with decreased Km 1-2 orders of magnitude and kcat 2-3 orders of magnitude | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
nitrocefin | wild type | Bacillus licheniformis | |
0.1 | - |
phenoxymethylpenicillin | wild type | Bacillus licheniformis | |
0.12 | - |
benzylpenicillin | wild type | Bacillus licheniformis | |
0.14 | - |
7-(thienyl-2-acetamido)-3-[2-(4-,N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid | wild type | Bacillus licheniformis | |
0.21 | - |
ampicillin | wild type | Bacillus licheniformis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus licheniformis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-(thienyl-2-acetamido)-3-[2-(4-N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid + H2O | i.e. PADAC | Bacillus licheniformis | (2R)-2-[(R)-carboxy{(E)-[1-hydroxy-2-(thiophen-2-yl)ethylidene]amino}methyl]-5-{[(2E)-2-{[4-(dimethyliminio)cyclohexa-2,5-dien-1-ylidene]hydrazinylidene}pyridin-1(2H)-yl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
ampicillin + H2O | - |
Bacillus licheniformis | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Bacillus licheniformis | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Bacillus licheniformis | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
phenoxymethylpenicillin + H2O | - |
Bacillus licheniformis | (2R,4S)-2-[(R)-carboxy(2-phenoxyacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | pH 7: stable up to 58, 62, 68°C for K234E, wild type and K234A respectively | Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
667 | - |
7-(thienyl-2-acetamido)-3-[2-(4-,N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid | - |
Bacillus licheniformis | |
1090 | - |
nitrocefin | - |
Bacillus licheniformis | |
2240 | - |
ampicillin | - |
Bacillus licheniformis | |
2550 | - |
phenoxymethylpenicillin | - |
Bacillus licheniformis | |
2650 | - |
benzylpenicillin | - |
Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
substrate phenoxymethylpenicillin | Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | substrate phenoxymethylpenicillin: kcat 1/6 at pH 9 compared to pH 6.5 | Bacillus licheniformis |