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Literature summary for 3.5.2.6 extracted from
- Enzymes from cold-adapted microorganisms: the class C beta-lactamase from antarctic psychrophile Psychrobacter immobilis A5 (1997), Eur. J. Biochem., 244, 186-191.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Psychrobacter immobilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Oxacillin | - |
Psychrobacter immobilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
benzylpenicillin | - |
Psychrobacter immobilis | |
| 0.006 | - |
ampicillin | - |
Psychrobacter immobilis | |
| 0.008 | - |
cephalexin | - |
Psychrobacter immobilis | |
| 0.051 | - |
nitrocefin | - |
Psychrobacter immobilis | |
| 0.1 | - |
cefazolin | - |
Psychrobacter immobilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Psychrobacter immobilis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SDS-PAGE | Psychrobacter immobilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Psychrobacter immobilis | - |
- |
- |
| Psychrobacter immobilis A5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Psychrobacter immobilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2094 | - |
- |
Psychrobacter immobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ampicillin + H2O | - |
Psychrobacter immobilis | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ampicillin + H2O | - |
Psychrobacter immobilis A5 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| benzylpenicillin + H2O | - |
Psychrobacter immobilis | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| benzylpenicillin + H2O | - |
Psychrobacter immobilis A5 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cefazolin + H2O | - |
Psychrobacter immobilis | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cefazolin + H2O | - |
Psychrobacter immobilis A5 | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalexin + H2O | - |
Psychrobacter immobilis | (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cephalexin + H2O | - |
Psychrobacter immobilis A5 | (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| nitrocefin + H2O | - |
Psychrobacter immobilis | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| nitrocefin + H2O | - |
Psychrobacter immobilis A5 | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| phenoxymethylpenicillin + H2O | - |
Psychrobacter immobilis | (2R,4S)-2-[(R)-carboxy(2-phenoxyacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Psychrobacter immobilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 6 | 40 | - |
Psychrobacter immobilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
ampicillin | - |
Psychrobacter immobilis | |
| 20 | - |
benzylpenicillin | - |
Psychrobacter immobilis | |
| 26 | - |
cephalexin | - |
Psychrobacter immobilis | |
| 476 | - |
cefazolin | - |
Psychrobacter immobilis | |
| 1410 | - |
nitrocefin | - |
Psychrobacter immobilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Psychrobacter immobilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | no activity below pH 6.5 | Psychrobacter immobilis |
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