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Literature summary for 3.5.2.6 extracted from

  • Vakulenko, S.B.; Taibi-Tronche, P.; Toth, M.; Massova, I.; Lerner, S.A.; Mobashery, S.
    Effects on substrate profile by mutational substitutions at position 164 and 179 of the class A TEMpUC19beta-lactamase from Escherichia coli (1999), J. Biol. Chem., 274, 23052-23060.
    View publication on PubMed
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D179G activity unchanged or enhanced Escherichia coli
D179N activity unchanged or enhanced Escherichia coli
R164N activity unchanged or enhanced Escherichia coli
R164S activity unchanged or enhanced Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 ampicillin
-
 Escherichia coli
0.07
-
 piperacillin
-
 Escherichia coli
0.12
-
 penicillin G
-
 Escherichia coli
0.2
-
 ceftazidime
-
 Escherichia coli
0.21
-
 cephalothin
-
 Escherichia coli
0.23
-
 cefotaxime
-
 Escherichia coli
0.24
-
 cefepime
-
 Escherichia coli
0.55
-
 aztreonam
-
 Escherichia coli
0.67
-
 cephaloridine
-
 Escherichia coli
0.95
-
 cephalosporin C
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli JM83
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Escherichia coli JM83 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
aztreonam + H2O
-
 Escherichia coli [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
aztreonam + H2O
-
 Escherichia coli JM83 [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
cefepime + H2O
-
 Escherichia coli (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephaloridine + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephaloridine + H2O
-
 Escherichia coli JM83 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephalosporin C + H2O
-
 Escherichia coli N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
cephalosporin C + H2O
-
 Escherichia coli JM83 N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
cephalothin + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephalothin + H2O
-
 Escherichia coli JM83 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
penicillin G + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
piperacillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00833
-
 ceftazidime
-
 Escherichia coli
0.183
-
 cefotaxime
-
 Escherichia coli
0.4
-
 aztreonam
-
 Escherichia coli
0.6
-
 cefepime
-
 Escherichia coli
33
-
 cephalosporin C
-
 Escherichia coli
41
-
 cephalothin
-
 Escherichia coli
770
-
 piperacillin
-
 Escherichia coli
780
-
 cephaloridine
-
 Escherichia coli
1040
-
 ampicillin
-
 Escherichia coli
1570
-
 penicillin G
-
 Escherichia coli