Cloned (Comment) | Organism |
---|---|
recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus licheniformis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml 14 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 20% w/v PEG 3350, and 0.1 M potassium thiocyanate, pH 6.5, equilibration against 0.5 ml reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51163 | - |
x * 51163, sequence calculation | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-allantoin + H2O | Bacillus licheniformis | allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective | allantoate | - |
r | |
(S)-allantoin + H2O | Bacillus licheniformis ATCC 14580 | allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective | allantoate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q65LN0 | - |
- |
Bacillus licheniformis ATCC 14580 | Q65LN0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, ultrafiltration, and dialysis | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-allantoin + H2O | allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective | Bacillus licheniformis | allantoate | - |
r | |
(S)-allantoin + H2O | allantoinase catalyzes the reversible hydrolysis of allantoin into allantoate by hydrolytic cleavage of the N1-C2 amide bond of the five-membered hydantoin ring. The enzyme shows an inverted enantioselectivity towards allantoin, R-enantioselective | Bacillus licheniformis ATCC 14580 | allantoate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 51163, sequence calculation | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
AllBali | - |
Bacillus licheniformis |