Literature summary for 3.5.2.3 extracted from

Lee, M.; Maher, M.J.; Guss, J.M.
Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-fluoroorotate: catalytic activity is reflected by the crystal form (2007), Acta Crystallogr. Sect. F, F63, 154-161.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with 5-fluoroorotate, hanging drop vapour diffusion method 14-16% PEG 3350, 0.1 M MES pH 6.25, 25 mM MgCl2, 0.2 M KCl and 30% sucrose	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
T109S	diminished activity	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
5-Fluoroorotate	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P05020	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-dihydroorotate + H2O	-	Escherichia coli	N-carbamoyl-L-aspartate	-	r
N-carbamoyl-L-aspartate	-	Escherichia coli	L-dihydroorotate + H2O	-	r

Synonyms

Synonyms	Comment	Organism
DHOase	-	Escherichia coli

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Release 2023.1 (January 2023)