Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109, the expression level of dihydropyrimidinase in the recombinant Escherichia coli cells is estimated to be about 20% of the respective total soluble proteins | Brevibacillus agri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a loss of 50% activity is observed in the presence of Zn2+ | Brevibacillus agri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the hydrolytic activity of PydB is enhanced notably by 0.5 mM Mn2+, about an 8fold increase in the enzymatic activity is observed in the presence of 0.05-5 mM Mn2+ | Brevibacillus agri | |
additional information | other divalent metal ions except Mn2+ and Zn2+ do not have significant effect on the enzyme activity | Brevibacillus agri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus agri | - |
- |
- |
Brevibacillus agri NCHU1002 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Brevibacillus agri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-5-(2-methylthioethyl)-hydantoin + H2O | the enzyme exhibits strict D-enantioselectivity for DL-5-(2-methylthioethyl)-hydantoin | Brevibacillus agri | N-carbamoyl-D-methionine | - |
? | |
DL-5-(2-methylthioethyl)-hydantoin + H2O | the enzyme exhibits strict D-enantioselectivity for DL-5-(2-methylthioethyl)-hydantoin | Brevibacillus agri NCHU1002 | N-carbamoyl-D-methionine | - |
? | |
DL-homophenylalanylhydantoin + H2O | the enzyme exhibits non-enantiospecificity for DL-homophenylalanylhydantoin | Brevibacillus agri | ? | - |
? | |
DL-homophenylalanylhydantoin + H2O | the enzyme exhibits non-enantiospecificity for DL-homophenylalanylhydantoin | Brevibacillus agri NCHU1002 | ? | - |
? | |
DL-p-hydroxyphenylhydantoin + H2O | the enzyme exhibits strict D-enantioselectivity for DL-p-hydroxyphenylhydantoin | Brevibacillus agri | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
DL-p-hydroxyphenylhydantoin + H2O | the enzyme exhibits strict D-enantioselectivity for DL-p-hydroxyphenylhydantoin | Brevibacillus agri NCHU1002 | N-carbamoyl-D-hydroxyphenylglycine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PydB | - |
Brevibacillus agri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Brevibacillus agri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | the enzyme is completely thermostable at 50°C for 20 days, and 50% of its initial activity remains after 36 days of incubation. After that, enzymatic activity gradually decreases to 4.4% after 65 days. Less than 15% maximum activity is observed at 90°C for 10 min. | Brevibacillus agri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in 50mM Tris-HCl buffer | Brevibacillus agri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | in 50mM Tris-HCl buffer | Brevibacillus agri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
the enzyme activity is completely lost below pH 5.5 | Brevibacillus agri |