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Literature summary for 3.5.2.2 extracted from

  • Lohkamp, B.; Andersen, B.; Piskur, J.; Dobritzsch, D.
    The crystal structures of dihydropyrimidinases reaffirm the close relationship between cyclic amidohydrolases and explain their substrate specificity (2006), J. Biol. Chem., 281, 13762-13776.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Lachancea kluyveri
-
Dictyostelium discoideum

Crystallization (Commentary)

Crystallization (Comment) Organism
equilibration, at 2.4 A resolution Lachancea kluyveri
with 100 mM Bistris, pH 6.5, 20% (w/v) PEG 3350, and 100 mM trisodium citrate Lachancea kluyveri
with 100 mM Bistris, pH 6.5, 20% (w/v) PEG 3350, and 100 mM trisodium citrate Dictyostelium discoideum
X-ray structure at at 2.05 A resolution Dictyostelium discoideum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ catalytic di-zinc center embedded by a (beta/alpha)8-barrel structural core, accompanied by a smaller beta-sandwich domain Lachancea kluyveri
Zn2+ catalytic di-zinc center embedded by a (beta/alpha)8-barrel structural core, accompanied by a smaller beta-sandwich domain Dictyostelium discoideum
Zn2+ contains a di-zinc center Lachancea kluyveri
Zn2+ contains a di-zinc center Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum Q55DL0
-
-
Lachancea kluyveri Q9P903
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lachancea kluyveri
-
Dictyostelium discoideum

Storage Stability

Storage Stability Organism
-20°C, HEPES buffer, pH 8.0, 3% glycerol Lachancea kluyveri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrothymine + H2O
-
Lachancea kluyveri 3-(carbamoylamino)-2-methylpropanoic acid
-
?
5,6-dihydrothymine + H2O
-
Dictyostelium discoideum 3-(carbamoylamino)-2-methylpropanoic acid
-
?
5,6-dihydrouracil + H2O
-
Lachancea kluyveri N-carbamoyl-beta-alanine
-
?
5,6-dihydrouracil + H2O
-
Dictyostelium discoideum N-carbamoyl-beta-alanine
-
?
5,6-dihydrouracil + H2O
-
Lachancea kluyveri N-carbamyl-beta-alanine
-
?
5,6-dihydrouracil + H2O
-
Dictyostelium discoideum N-carbamyl-beta-alanine
-
?
N-carbamoyl-beta-alanine + H2O
-
Lachancea kluyveri ?
-
?
N-carbamoyl-beta-alanine + H2O
-
Dictyostelium discoideum ?
-
?

Subunits

Subunits Comment Organism
homotetramer structure solved by molecular replacement, corresponding to the native state of the enzyme in solution Lachancea kluyveri
tetramer
-
Dictyostelium discoideum
tetramer x-ray crystallography Lachancea kluyveri
tetramer x-ray crystallography Dictyostelium discoideum

Synonyms

Synonyms Comment Organism
DHPase
-
Lachancea kluyveri
DHPase
-
Dictyostelium discoideum
dihydropyrimidine amidohydrolase
-
Lachancea kluyveri
dihydropyrimidine amidohydrolase
-
Dictyostelium discoideum