Literature summary for 3.5.2.2 extracted from

Werner, M.; Vasquez, F.J.; Fritz, C.; Vielhauer, O.; Siemann-Herzberg, M.; Altenbucher, J.; Syldatk, C.
Cloning of D-specific hydantoin utilization genes from Arthrobacter crystallopoietes (2004), Eng. Life Sci., 4, 563-572.

No PubMed abstract available

Search for more literature for 3.5.2.2

Application

Application	Comment	Organism
additional information	useful for industrial production of enantiomerically pure D-amino acids	Arthrobacter crystallopoietes

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli JM109	Arthrobacter crystallopoietes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	increases activity, when DL-benzyl-hydantoin is used as substrate	Arthrobacter crystallopoietes
additional information	no increase of activity by Fe2+, Mg2+ and Mn2+	Arthrobacter crystallopoietes
Zn2+	increases activity, when DL-benzyl-hydantoin is used as substrate	Arthrobacter crystallopoietes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49290	-	calculated from sequence data	Arthrobacter crystallopoietes
54000	-	purified enzyme	Arthrobacter crystallopoietes
55000	-	recombinant enzyme, SDS-PAGE	Arthrobacter crystallopoietes

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter crystallopoietes	Q84FR6	strain DSM20117	-
Arthrobacter crystallopoietes DSM 20117	Q84FR6	strain DSM20117	-
Geobacillus stearothermophilus	Q45515	-	-

Purification (Commentary)

Purification (Comment)	Organism
enzyme from wild type 19.8fold purified after MonoQ chromatography, recombinant enzyme from Escherichia coli JM109pJAVI2 90% purified by Strep-tag technology	Arthrobacter crystallopoietes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.93	-	purified recombinant enzyme from Escherichia coli JM109pJAVI2	Arthrobacter crystallopoietes

Storage Stability

Storage Stability	Organism
-20°C, lactate medium	Arthrobacter crystallopoietes

Synonyms

Synonyms	Comment	Organism
D-hydantoinase	-	Geobacillus stearothermophilus
D-hydantoinase	-	Arthrobacter crystallopoietes
hydantoinase	-	Geobacillus stearothermophilus
hydantoinase	-	Arthrobacter crystallopoietes
hyuH	-	Arthrobacter crystallopoietes

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Release 2023.1 (January 2023)