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Literature summary for 3.5.2.2 extracted from

  • Dobritzsch, D.; Andersen, B.; Piskur, J.
    Crystallization and X-ray diffraction analysis of dihydropyrimidinase from Saccharomyces kluyveri (2005), Acta Crystallogr. Sect. F, 61, 359-362.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression as His8-tagged protein in Escherichia coli BL21 Lachancea kluyveri

Crystallization (Commentary)

Crystallization (Comment) Organism
hangin-drop vapour-diffusion method Lachancea kluyveri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62300
-
4 * 62300, His8-tagged protein Lachancea kluyveri

Organism

Organism UniProt Comment Textmining
Lachancea kluyveri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Lachancea kluyveri

Storage Stability

Storage Stability Organism
-20°C, Tris buffer, pH 7.5, shorter times Lachancea kluyveri
-80°C, Tris buffer, pH 7.5, longer times Lachancea kluyveri

Subunits

Subunits Comment Organism
homotetramer 4 * 62300, His8-tagged protein Lachancea kluyveri

Synonyms

Synonyms Comment Organism
DHPase
-
Lachancea kluyveri