Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.2.2 extracted from

  • Xu, Z.; Liu, Y.; Yang, Y.; Jiang, W.; Arnold, E.; Ding, J.
    Crystal structure of D-hydantoinase from Burkholderia pickettii at a resolution of 2.7 Angstroms: insights into the molecular basis of enzyme thermostability (2003), J. Bacteriol., 185, 4038-4049.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.2

Application

Application Comment Organism
synthesis industrial enzyme, that is widely used in the production of D-amino acids which are precursors for semisynthesis of antibiotics, peptides, and pesticides Ralstonia pickettii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ralstonia pickettii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method, crystal structure at 2.7 A resolution Ralstonia pickettii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates Ralstonia pickettii
Fe2+ stimulates Ralstonia pickettii
Mn2+ stimulates Ralstonia pickettii
Ni2+ stimulates Ralstonia pickettii
Zn2+ stimulates Ralstonia pickettii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 2 * 52000, reducing and nonreducing SDS-PAGE Ralstonia pickettii
52253
-
 2 * 52253, mass spectrometric analysis Ralstonia pickettii
105000
-
 gel filtration Ralstonia pickettii
110000
-
 dynamic light scattering analysis Ralstonia pickettii

Organism

Organism UniProt Comment Textmining
Ralstonia pickettii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ralstonia pickettii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00113
-
-
 Ralstonia pickettii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-hydroxyphenylhydantoin + H2O
-
 Ralstonia pickettii N-carbamoyl-D-hydroxyphenylglycine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 52000, reducing and nonreducing SDS-PAGE Ralstonia pickettii
dimer 2 * 52253, mass spectrometric analysis Ralstonia pickettii

Synonyms

Synonyms Comment Organism
D-HYD
-
 Ralstonia pickettii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Ralstonia pickettii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, stable Ralstonia pickettii
50
-
 30 min, 11% loss of activity in absence of metal ions or in presence of Co2+ or Mn2+ Ralstonia pickettii
60
-
 30 min, 65% loss of activity in absence of metal ions, 20% loss of activity in presence of Co2+ or Mn2+ Ralstonia pickettii
70
-
 30 min, complete loss of activity above, in absence of metal ions. 85% loss of activity in presence of Co2+ or Mn2+ Ralstonia pickettii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Ralstonia pickettii