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Literature summary for 3.5.2.2 extracted from
- Optimization of the enzymatic synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin using D-hydantoinase and N-carbamoylase (1995), Enzyme Microb. Technol., 17, 63-67.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides | Agrobacterium sp. |
| medicine | development of the enzymatic process for production of D-p-hydroxyphenylglycine from DL-5-hydroxyphenylhydantoin, new drugs based on p-hydroxyphenylglycine like aspoxicillin, cefbuperazine and cepyramide expected to be marketed in near future | Agrobacterium sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.28 | - |
hydantoin | - |
Agrobacterium sp. |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydantoin + H2O | Agrobacterium tumefaciens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. IP-I 671 | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. 1-671 | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium sp. | - |
- |
- |
| Agrobacterium sp. IP-I 671 | - |
- |
- |
| Agrobacterium tumefaciens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Agrobacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.3 | - |
- |
Agrobacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Agrobacterium tumefaciens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. IP-I 671 | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. 1-671 | 3-ureidopropionate | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. 1-671 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. 1-671 | N-carbamoyl glycine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Agrobacterium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
highly denatured at temperatures above | Agrobacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Agrobacterium sp. |
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