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Literature summary for 3.5.2.2 extracted from
- A thermostable D-hydantoinase isolated from a mesophilic Bacillus sp. AR9 (1997), Biochem. Biophys. Res. Commun., 234, 485-488.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Bos taurus |  |
| Co2+ | - |
Geobacillus stearothermophilus | |
| Co2+ | enhances activity by more than 60% | Bacillus sp. (in: Bacteria) | |
| Fe2+ | - |
Pseudomonas putida | |
| Mg2+ | - |
Bos taurus | |
| Mg2+ | - |
Bacillus sp. (in: Bacteria) | |
| Mn2+ | - |
Bos taurus | |
| Mn2+ | - |
Geobacillus stearothermophilus | |
| Mn2+ | - |
Bacillus sp. (in: Bacteria) | |
| Ni2+ | - |
Bacillus sp. (in: Bacteria) | |
| Ni2+ | - |
Agrobacterium sp. | |
| Zn2+ | - |
Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrothymine + H2O | Bos taurus | - |
? | - |
? | |
| 5,6-dihydrothymine + H2O | Bacillus sp. (in: Bacteria) | - |
? | - |
? | |
| 5,6-dihydrothymine + H2O | Bacillus sp. (in: Bacteria) AR9 | - |
? | - |
? | |
| 5,6-dihydrouracil + H2O | Bos taurus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Geobacillus stearothermophilus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Pseudomonas putida | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Bacillus sp. (in: Bacteria) | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Agrobacterium tumefaciens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Peptococcus anaerobius | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Geobacillus stearothermophilus SD-1 | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Bacillus sp. (in: Bacteria) AR9 | - |
3-ureidopropionate | - |
? | |
| hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Bacillus sp. (in: Bacteria) | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium tumefaciens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Peptococcus anaerobius | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus SD-1 | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium sp. | - |
- |
- |
| Agrobacterium tumefaciens | - |
- |
- |
| Bacillus sp. (in: Bacteria) | - |
AR9 | - |
| Bacillus sp. (in: Bacteria) AR9 | - |
AR9 | - |
| Bos taurus | - |
bovine | - |
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus SD-1 | - |
- |
- |
| Peptococcus anaerobius | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrothymine + H2O | - |
Bos taurus | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 5,6-dihydrothymine + H2O | - |
Bacillus sp. (in: Bacteria) AR9 | ? | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Bacillus sp. (in: Bacteria) | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium tumefaciens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Peptococcus anaerobius | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus SD-1 | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Bacillus sp. (in: Bacteria) AR9 | 3-ureidopropionate | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Bos taurus | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium tumefaciens | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) AR9 | ? | - |
? | |
| DL-5-methylhydantoin | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| DL-5-methylhydantoin | - |
Agrobacterium tumefaciens | ? | - |
? | |
| hydantoin + H2O | - |
Bos taurus | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Peptococcus anaerobius | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) AR9 | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Bacillus sp. (in: Bacteria) | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Peptococcus anaerobius | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl glycine | - |
? | |
| additional information | - |
Bos taurus | ? | - |
? | |
| additional information | - |
Agrobacterium tumefaciens | ? | - |
? | |
| additional information | DL-hydroxymethylhydantoin and 5,5-disubstituted hydantoin derivatives are no substrates | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| additional information | DL-hydroxymethylhydantoin and 5,5-disubstituted hydantoin derivatives are no substrates | Bacillus sp. (in: Bacteria) AR9 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Pseudomonas sp. |
| 65 | - |
- |
Geobacillus stearothermophilus |
| 65 | - |
- |
Bacillus sp. (in: Bacteria) |
| 70 | - |
- |
Geobacillus stearothermophilus |
| 70 | - |
- |
Agrobacterium tumefaciens |
| 70 | - |
- |
Agrobacterium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable up to | Pseudomonas sp. |
| 60 | - |
- |
Geobacillus stearothermophilus |
| 60 | - |
half-life of 1 h | Peptococcus anaerobius |
| 70 | - |
half-life of 80 min, loses only 33% activity in 4 h at 60°C, retains 90% activity in the range 55-70°C, half-life of more than 12 h at 50°C, retains more than 70% activity at 60°C | Bacillus sp. (in: Bacteria) |
| 70 | - |
retains 30% of its activity in 30 min | Agrobacterium sp. |
| 80 | - |
half-life of 20 min | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Geobacillus stearothermophilus |
| 7 | 8 | - |
Pseudomonas sp. |
| 9.5 | - |
- |
Geobacillus stearothermophilus |
| 9.5 | - |
- |
Bacillus sp. (in: Bacteria) |
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