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Literature summary for 3.5.2.2 extracted from
- Purification and characterization of thermostable D-hydantoinase from Bacillus thermocatenulatus GH-2 (1999), Appl. Biochem. Biotechnol., 81, 53-64.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Uracil | induces biosynthesis of enzyme | Geobacillus thermocatenulatus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Geobacillus stearothermophilus |
| synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Geobacillus thermocatenulatus |
| synthesis | thermostable enzyme with great biotechnological potential | Geobacillus thermocatenulatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30.7 | - |
p-hydroxyphenylhydantoin | - |
Geobacillus thermocatenulatus | |
| 47.2 | - |
p-hydroxyphenylhydantoin | - |
Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Geobacillus stearothermophilus | |
| Co2+ | - |
Geobacillus thermocatenulatus | |
| Fe2+ | - |
Geobacillus thermocatenulatus | |
| Mn2+ | - |
Geobacillus stearothermophilus | |
| Mn2+ | - |
Geobacillus thermocatenulatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
4 * 56000, SDS-PAGE | Geobacillus thermocatenulatus |
| 120000 | - |
strain SD-1 | Geobacillus stearothermophilus |
| 200000 | - |
strain NS1122A | Geobacillus stearothermophilus |
| 230000 | - |
gel filtration | Geobacillus thermocatenulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus thermocatenulatus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus SD-1 | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium sp. | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus | - |
strains NS1122A and LU1220 | - |
| Geobacillus stearothermophilus SD-1 | - |
- |
- |
| Geobacillus thermocatenulatus | - |
- |
- |
| Geobacillus thermocatenulatus GH-2 | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus thermocatenulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.9 | - |
isopropylhydantoin as substrate | Geobacillus thermocatenulatus |
| 27.5 | - |
dihydrouracil as substrate | Geobacillus thermocatenulatus |
| 91.6 | - |
hydroxyphenylhydantoin as substrate | Geobacillus thermocatenulatus |
| 101.5 | - |
hydantoin as substrate | Geobacillus thermocatenulatus |
| 236.9 | - |
phenylhydantoin as substrate | Geobacillus thermocatenulatus |
| 461 | - |
- |
Geobacillus thermocatenulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium sp. | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus thermocatenulatus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus SD-1 | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus thermocatenulatus GH-2 | 3-ureidopropionate | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | N-carbamoyl-D-valine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | N-carbamoyl-D-phenylglycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus thermocatenulatus | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus thermocatenulatus GH-2 | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus thermocatenulatus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl glycine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Geobacillus stearothermophilus |
| tetramer | 4 * 56000, SDS-PAGE | Geobacillus thermocatenulatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Geobacillus stearothermophilus |
| 65 | - |
- |
Geobacillus thermocatenulatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | - |
Geobacillus thermocatenulatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Geobacillus stearothermophilus |
| 80 | - |
half-life about 45 min, stable up to 75°C | Geobacillus thermocatenulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Geobacillus thermocatenulatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | - |
Geobacillus thermocatenulatus |
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