Application | Comment | Organism |
---|---|---|
synthesis | - |
Thermus sp. |
synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
D-hydantoinase genes cloned and sequenced, Escherichia coli transformed with cosmid vectors, D-hydantoinase expressed and identified | Thermus sp. |
expressed in Escherichia coli BL21 | Thermus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Geobacillus stearothermophilus | |
Cu2+ | - |
Pseudomonas fluorescens | |
N-Carbamoyl-glycine | competitive inhibition | Geobacillus stearothermophilus | |
Zn2+ | - |
Geobacillus stearothermophilus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
dihydrouracil | - |
Geobacillus stearothermophilus | |
32.1 | - |
phenylhydantoin | - |
Geobacillus stearothermophilus | |
47.2 | - |
p-hydroxyphenylhydantoin | - |
Geobacillus stearothermophilus | |
340 | - |
hydantoin | - |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Bos taurus | |
Mn2+ | - |
Thermus sp. | |
Mn2+ | requires 1 ion per molecule of enzyme for activity | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51050 | - |
determined by mass spectrometer | Geobacillus stearothermophilus |
51100 | - |
2 * 51100, mass spectrometric analysis | Geobacillus stearothermophilus |
54000 | - |
2 * 54000, SDS-PAGE | Geobacillus stearothermophilus |
102100 | - |
mass spectrometric analysis | Geobacillus stearothermophilus |
133900 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + H2O | Bos taurus | - |
3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | Geobacillus stearothermophilus | - |
3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | Pseudomonas fluorescens | - |
3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | Pseudomonas putida | - |
3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | Thermus sp. | - |
3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | Geobacillus stearothermophilus SD-1 | - |
3-ureidopropionate | - |
? | |
hydantoin + H2O | Rattus norvegicus | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Pseudomonas fluorescens | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Thermus sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Geobacillus stearothermophilus SD-1 | - |
N-carbamoyl glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium sp. | - |
- |
- |
Bos taurus | - |
calf | - |
Bos taurus | - |
bovine | - |
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus SD-1 | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Thermus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
- |
Pseudomonas fluorescens |
- |
Pseudomonas putida |
- |
Agrobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
148.6 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + H2O | - |
Rattus norvegicus | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Pseudomonas fluorescens | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Thermus sp. | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Agrobacterium sp. | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus SD-1 | 3-ureidopropionate | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Bos taurus | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-phenylglycine | - |
? | |
DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl-D-phenylglycine | - |
? | |
hydantoin + H2O | - |
Bos taurus | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Pseudomonas fluorescens | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Thermus sp. | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Rattus norvegicus | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Thermus sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus SD-1 | N-carbamoyl glycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Thermus sp. |
dimer | 2 * 54000, SDS-PAGE | Geobacillus stearothermophilus |
dimer | 2 * 51100, mass spectrometric analysis | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
hydantoin as substrate | Geobacillus stearothermophilus |
70 | - |
- |
Agrobacterium sp. |
70 | - |
DL-p-hydroxyphenylhydantoin as substrate | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pseudomonas putida |
60 | - |
stable up to | Geobacillus stearothermophilus |
70 | - |
treatment for 30 min, 20% activity remains | Agrobacterium sp. |
80 | - |
half-life 30 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus stearothermophilus |
8 | - |
- |
Pseudomonas fluorescens |
8.8 | - |
- |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
remains stable at pH values higher than | Geobacillus stearothermophilus |