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Literature summary for 3.5.2.2 extracted from
- Purification and characterization of the D-hydantoinase from Bacillus circulans (1997), Appl. Biochem. Biotechnol., 62, 219-230.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Geobacillus stearothermophilus |
| synthesis | commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals | Niallia circulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus |  |
| p-hydroxymercuribenzoate | - |
Niallia circulans | |
| p-hydroxymercuribenzoate | - |
Pseudomonas fluorescens | |
| p-hydroxymercuribenzoate | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.9 | - |
DL-5-isobutylhydantoin | - |
Niallia circulans | |
| 5.8 | - |
allantoin | - |
Niallia circulans | |
| 6.8 | - |
DL-5-benzylhydantoin | - |
Niallia circulans | |
| 12.8 | - |
DL-5-butylhydantoin | - |
Niallia circulans | |
| 17.2 | - |
DL-5-isopropylhydantoin | - |
Niallia circulans | |
| 25.6 | - |
DL-5-propylhydantoin | - |
Niallia circulans | |
| 27.8 | - |
DL-5-methylhydantoin | - |
Niallia circulans | |
| 40.7 | - |
DL-5-(2-methylthioethyl)hydantoin | - |
Niallia circulans | |
| 58.8 | - |
DL-5-phenyllhydantoin | - |
Niallia circulans | |
| 105.4 | - |
hydantoin | - |
Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
4 * 53000, SDS-PAGE | Niallia circulans |
| 54000 | - |
4 * 54000, gel filtration, denaturing conditions | Niallia circulans |
| 212000 | - |
gel filtration | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydantoin + H2O | Brevibacillus brevis | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas fluorescens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Niallia circulans | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Arthrobacter crystallopoietes | - |
N-carbamoyl glycine | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| SDS | - |
Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium sp. | - |
- |
- |
| Agrobacterium sp. IP-I 671 | - |
- |
- |
| Arthrobacter crystallopoietes | - |
- |
- |
| Brevibacillus brevis | - |
L-specific hydantoinase | - |
| Geobacillus stearothermophilus | - |
- |
- |
| Niallia circulans | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Niallia circulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43.8 | - |
- |
Niallia circulans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, 50% initial activity left after 6 months | Niallia circulans |
| 4°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, retains full activity 1 month | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allantoin + H2O | - |
Niallia circulans | ? | - |
? | |
| allantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| allantoin + H2O | - |
Agrobacterium sp. IP-I 671 | ? | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Niallia circulans | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Arthrobacter crystallopoietes | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | N-carbamoyl-D-methionine | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Pseudomonas putida | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Niallia circulans | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Arthrobacter crystallopoietes | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | ? | - |
? | |
| DL-5-butylhydantoin | - |
Niallia circulans | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Niallia circulans | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium sp. | ? | - |
? | |
| DL-5-isobutyl-hydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | ? | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Niallia circulans | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl-D-valine | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Agrobacterium sp. IP-I 671 | N-carbamoyl-D-valine | - |
? | |
| DL-5-methylhydantoin | - |
Niallia circulans | ? | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Niallia circulans | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Arthrobacter crystallopoietes | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-propylhydantoin | - |
Niallia circulans | ? | - |
? | |
| hydantoin + H2O | - |
Brevibacillus brevis | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Niallia circulans | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Arthrobacter crystallopoietes | N-carbamoyl glycine | - |
? | |
| additional information | - |
Pseudomonas putida | ? | - |
? | |
| additional information | 5,5-dimethylhydantoin is no substrate | Niallia circulans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Geobacillus stearothermophilus |
| tetramer | - |
Pseudomonas fluorescens |
| tetramer | - |
Arthrobacter crystallopoietes |
| tetramer | 4 * 53000, SDS-PAGE | Niallia circulans |
| tetramer | 4 * 54000, gel filtration, denaturing conditions | Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
DL-5-isopropylhydantoin as substrate | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Geobacillus stearothermophilus |
| 60 | - |
- |
Pseudomonas putida |
| 60 | - |
stable up to, retains full activity on heating at 40°C for 8 h, retains 75% original activity at 62.5°C for 8 h | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Niallia circulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | most stable in this range | Niallia circulans |
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