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Literature summary for 3.5.2.2 extracted from
- Characterization of hydantoinase from Pseudomonas fluorescens strain DSM 84 (1986), Biotechnol. Appl. Biochem., 8, 564-574.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | - |
Pseudomonas putida |
| nutrition | useful for production of D-amino acids, microbial hydantoinases used industrially to produce N-carbamoyl-D-hydroxyphenylglycine | Pseudomonas fluorescens |
| nutrition | useful for production of D-amino acids, microbial hydantoinases used industrially to produce N-carbamoyl-D-hydroxyphenylglycine | Pseudomonas putida |
General Stability
| General Stability | Organism |
|---|---|
| fairly stable during storage in appropriate buffers | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Pseudomonas fluorescens |  |
| 2,2'-dipyridyl | - |
Pseudomonas putida | |
| 8-hydroxyquinoline | - |
Pseudomonas fluorescens | |
| 8-hydroxyquinoline | - |
Pseudomonas putida | |
| Cu2+ | - |
Pseudomonas fluorescens | |
| Cu2+ | - |
Pseudomonas putida | |
| p-hydroxymercuribenzoate | - |
Pseudomonas fluorescens | |
| p-hydroxymercuribenzoate | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
5,6-dihydrouracil | - |
Pseudomonas fluorescens | |
| 34 | - |
5-isopropylhydantoin | - |
Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Pseudomonas putida | |
| Fe2+ | needs ferrous ions for activity or reactivation, pure enzyme up to 5 times more active in presence of Fe2+ | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE | Pseudomonas fluorescens |
| 230000 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | Pseudomonas fluorescens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Pseudomonas putida | - |
3-ureidopropionate | - |
? | |
| hydantoin + H2O | Pseudomonas fluorescens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
strain DSM 84 | - |
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.44 | - |
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Pseudomonas fluorescens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
| 5-(p-hydroxybenzyl)hydantoin + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
| 5-(p-hydroxybenzyl)hydantoin + H2O | - |
Pseudomonas putida | ? | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-methionine | - |
? | |
| DL-5-benzylhydantoin + H2O | - |
Pseudomonas putida | ? | - |
? | |
| DL-5-benzylhydantoin + H2O | poorly hydrolyzed | Pseudomonas fluorescens | ? | - |
? | |
| DL-5-indolylmethylhydantoin + H2O | - |
Pseudomonas fluorescens | L-tryptophan | - |
? | |
| DL-5-isopropylhydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl-D-valine | - |
? | |
| hydantoin + H2O | poorly hydrolyzed | Pseudomonas fluorescens | hydantoic acid | - |
? | |
| hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Pseudomonas fluorescens |
| 55 | - |
- |
Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | - |
Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable at temperatures up to, starts to lose significant activity at 50°C, remaining activity at 55°C less than 25%, heat treatment in presence of substrate stabilizes enzyme at temperatures in excess of 40°C | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
dihydrouracil as substrate | Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | - |
Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Pseudomonas putida |
| 5.5 | 8.5 | most stable in this range, more stable at alkaline pH than at acid pH | Pseudomonas fluorescens |
| 6 | 9.5 | enzyme inactivated at pH 3.5-4 and above pH 10.5 | Pseudomonas fluorescens |
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