Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta 2(DE3)pLysS | Oncorhynchus mykiss |
expression in Escherichia coli Rosetta 2(DE3)pLysS | Branchiostoma japonicum |
Protein Variants | Comment | Organism |
---|---|---|
R54E/Y119T | amino acid substitutions, R54E/Y119T, at the active sites of HIUHase, exert weak [125I]-3,3',5-triiodo-l-thyronine([125I]T3) binding activity with a concomitant loss of 5-hydroxyisourate hydrolysis activity | Oncorhynchus mykiss |
R54E/Y119T | amino acid substitutions, R54E/Y119T, at the active sites of HIUHase, exert weak [125I]-3,3',5-triiodo-l-thyronine([125I]T3) binding activity with a concomitant loss of 5-hydroxyisourate hydrolysis activity | Branchiostoma japonicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of 3His to the N-terminal end weakens 5-hydroxyisourate hydrolysis activity, whereas it enhanced T3-binding activity of R54E/Y119T mutsant enzyme | Branchiostoma japonicum | |
additional information | addition of 3His to the N-terminal end weakens 5-hydroxyisourate hydrolysis activity, whereas it enhanced T3-binding activity of R54E/Y119T mutsant enzyme | Oncorhynchus mykiss |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyisourate + H2O | Oncorhynchus mykiss | - |
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
5-hydroxyisourate + H2O | Branchiostoma japonicum | - |
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Branchiostoma japonicum | K9MS40 | - |
- |
Oncorhynchus mykiss | K9MS40 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Oncorhynchus mykiss |
- |
Branchiostoma japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyisourate + H2O | - |
Oncorhynchus mykiss | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
5-hydroxyisourate + H2O | - |
Branchiostoma japonicum | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-hydroxyisourate hydrolase | - |
Oncorhynchus mykiss |
5-hydroxyisourate hydrolase | - |
Branchiostoma japonicum |
HIUHase | - |
Oncorhynchus mykiss |
HIUHase | - |
Branchiostoma japonicum |
General Information | Comment | Organism |
---|---|---|
evolution | sequential molecular events of functional trade-offs in 5-hydroxyisourate hydrolase before and after gene duplication led to the evolution of transthyretin during chordate diversification | Oncorhynchus mykiss |
evolution | sequential molecular events of functional trade-offs in 5-hydroxyisourate hydrolase before and after gene duplication led to the evolution of transthyretin during chordate diversification | Branchiostoma japonicum |
metabolism | the enzyme is involved in urate metabolism | Oncorhynchus mykiss |
metabolism | the enzyme is involved in urate metabolism | Branchiostoma japonicum |