BRENDA - Enzyme Database
show all sequences of 3.5.2.17

A transthyretin-related protein is functionally expressed in Herbaspirillum seropedicae

Matiollo, C.; Vernal, J.; Ecco, G.; Bertoldo, J.B.; Razzera, G.; de Souza, E.M.; Pedrosa, F.O.; Terenzi, H.; Biochem. Biophys. Res. Commun. 387, 712-716 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Herbaspirillum seropedicae
Engineering
Protein Variants
Commentary
Organism
H101A
10% of wild-type activity
Herbaspirillum seropedicae
H7A
3% of wild-type activity
Herbaspirillum seropedicae
Y114F
22% of wild-type activity
Herbaspirillum seropedicae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12439
-
x * 12439, calculated, x * 15000, SDS-PAGE of recombinant protein
Herbaspirillum seropedicae
15000
-
x * 12439, calculated, x * 15000, SDS-PAGE of recombinant protein
Herbaspirillum seropedicae
Organism
Organism
UniProt
Commentary
Textmining
Herbaspirillum seropedicae
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
37650
-
pH 7.0, 25░C
Herbaspirillum seropedicae
Subunits
Subunits
Commentary
Organism
?
x * 12439, calculated, x * 15000, SDS-PAGE of recombinant protein
Herbaspirillum seropedicae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Herbaspirillum seropedicae
Engineering (protein specific)
Protein Variants
Commentary
Organism
H101A
10% of wild-type activity
Herbaspirillum seropedicae
H7A
3% of wild-type activity
Herbaspirillum seropedicae
Y114F
22% of wild-type activity
Herbaspirillum seropedicae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12439
-
x * 12439, calculated, x * 15000, SDS-PAGE of recombinant protein
Herbaspirillum seropedicae
15000
-
x * 12439, calculated, x * 15000, SDS-PAGE of recombinant protein
Herbaspirillum seropedicae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
37650
-
pH 7.0, 25░C
Herbaspirillum seropedicae
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 12439, calculated, x * 15000, SDS-PAGE of recombinant protein
Herbaspirillum seropedicae
Other publictions for EC 3.5.2.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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644454
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2003
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644456
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