BRENDA - Enzyme Database
show all sequences of 3.5.2.17

Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzyme

Sarma, A.D.; Serfozo, P.; Kahn, K.; Tipton, P.A.; J. Biol. Chem. 274, 33863-33865 (1999)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
5-hydroxyisourate
pH 7.2, 25°C
Glycine max
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
gel filtration
Glycine max
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-hydroxyisourate + H2O
Glycine max
-
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
-
; soybean
-
Purification (Commentary)
Commentary
Organism
-
Glycine max
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10
-
-
Glycine max
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxyisourate + H2O
-
644456
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
-
?
5-hydroxyisourate + H2O
-
644456
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
644456
Glycine max
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 68000, SDS-PAGE
Glycine max
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11
-
5-hydroxyisourate
pH 7.2, 25°C
Glycine max
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
5-hydroxyisourate
pH 7.2, 25°C
Glycine max
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
gel filtration
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-hydroxyisourate + H2O
Glycine max
-
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Glycine max
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10
-
-
Glycine max
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxyisourate + H2O
-
644456
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
-
?
5-hydroxyisourate + H2O
-
644456
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
644456
Glycine max
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 68000, SDS-PAGE
Glycine max
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11
-
5-hydroxyisourate
pH 7.2, 25°C
Glycine max
Other publictions for EC 3.5.2.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2
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9
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1
1
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1
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1
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1
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1
2
1
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1
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9
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1
1
1
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1
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2
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1
1
4
-
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5
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3
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4
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5
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1
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1
4
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5
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4
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5
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5
5
720251
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711614
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2
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3
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1
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1
1
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713417
Stevenson
Deficiency of 5-hydroxyisourat ...
Mus musculus
Proc. Natl. Acad. Sci. USA
107
16625-16630
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1
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1
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4
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1
1
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697901
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Chilotilapia rhoadesii, Escherichia coli, Homo sapiens
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276
1999-2011
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3
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1
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7
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3
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3
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3
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3
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3
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3
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1
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3
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3
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3
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3
3
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711070
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Herbaspirillum seropedicae
Biochem. Biophys. Res. Commun.
387
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2009
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1
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3
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2
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4
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1
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1
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1
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3
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2
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1
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1
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666761
Jung
Structural and functional anal ...
Bacillus subtilis
Proc. Natl. Acad. Sci. USA
103
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2006
-
-
1
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3
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1
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1
1
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1
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1
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1
1
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688334
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The crystal structure of the t ...
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J. Mol. Biol.
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2006
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1
1
3
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6
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1
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1
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1
1
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3
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1
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1
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1
1
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688335
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2006
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-
1
1
1
-
-
-
-
-
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2
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1
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1
1
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1
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1
1
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1
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1
1
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688940
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6
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6
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1
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1
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1
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6
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1
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1
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644454
Raychaudhuri
A familiar motif in a new cont ...
Glycine max
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42
6848-6852
2003
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1
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2
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2
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2
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2
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1
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4
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2
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1
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2
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2
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1
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4
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2
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644455
Raychaudhuri
Cloning and expression of the ...
Glycine max
Plant Physiol.
130
2061-2068
2002
-
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1
1
2
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1
1
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2
1
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5
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1
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3
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1
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1
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2
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1
1
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1
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1
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3
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1
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644456
Sarma
Identification and purificatio ...
Glycine max
J. Biol. Chem.
274
33863-33865
1999
-
-
-
-
-
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1
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1
1
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2
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1
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1
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2
1
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1
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1
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1
1
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1
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1
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1
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