BRENDA - Enzyme Database
show all sequences of 3.5.2.17

Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism

Raychaudhuri, A.; Tipton, P.A.; Plant Physiol. 130, 2061-2068 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloned and expressed in Escherichia coli
Glycine max
Crystallization (Commentary)
Crystallization
Organism
-
Glycine max
Engineering
Amino acid exchange
Commentary
Organism
E199A
site-directed mutagenesis, mutant devoid of detectable catalytic activity
Glycine max
E408A
site-directed mutagenesis, mutant devoid of detectable catalytic activity
Glycine max
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
5-hydroxyisourate
pH 7.5, 37°C, recombinant enzyme lacking the signal peptide
Glycine max
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
-
Glycine max
5777
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63730
-
calculated from cDNA sequence
Glycine max
68000
-
SDS-PAGE, gel filtration
Glycine max
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-hydroxyisourate + H2O
Glycine max
-
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
-
-
-
Glycine max
Q8S3J3
soybean, cv Williams
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Glycine max
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-hydroxyisourate + H2O
-
644455
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
-
-
5-hydroxyisourate + H2O
-
644455
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
-
?
5-hydroxyisourate + H2O
-
644455
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
644455
Glycine max
?
Synonyms
Synonyms
Commentary
Organism
HIUHase
-
Glycine max
hydroxyisourate hydrolase
-
Glycine max
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
5-hydroxyisourate
pH 7.5, 37°C, recombinant enzyme lacking the signal peptide
Glycine max
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned and expressed in Escherichia coli
Glycine max
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Glycine max
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E199A
site-directed mutagenesis, mutant devoid of detectable catalytic activity
Glycine max
E408A
site-directed mutagenesis, mutant devoid of detectable catalytic activity
Glycine max
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
5-hydroxyisourate
pH 7.5, 37°C, recombinant enzyme lacking the signal peptide
Glycine max
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
-
Glycine max
5777
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63730
-
calculated from cDNA sequence
Glycine max
68000
-
SDS-PAGE, gel filtration
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-hydroxyisourate + H2O
Glycine max
-
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Glycine max
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-hydroxyisourate + H2O
-
644455
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
-
-
5-hydroxyisourate + H2O
-
644455
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
-
-
?
5-hydroxyisourate + H2O
-
644455
Glycine max
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
644455
Glycine max
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
5-hydroxyisourate
pH 7.5, 37°C, recombinant enzyme lacking the signal peptide
Glycine max
Other publictions for EC 3.5.2.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733937
Kasai
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1
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1
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1
2
1
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1
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9
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1
1
3
1
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1
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1
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1
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1
2
1
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1
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9
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1
1
1
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1
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2
2
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-
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718476
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Structural and kinetic insight ...
Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 700721
Acta Crystallogr. Sect. D
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1
1
4
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5
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3
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1
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5
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1
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1
4
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5
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4
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5
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5
5
720251
Cendron
Probing the evolution of hydro ...
Danio rerio
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1
3
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711614
Pessoa
Functional characterization of ...
Arabidopsis thaliana
BMC Plant Biol.
10
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1
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2
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1
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1
1
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713417
Stevenson
Deficiency of 5-hydroxyisourat ...
Mus musculus
Proc. Natl. Acad. Sci. USA
107
16625-16630
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1
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1
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4
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1
1
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697901
Lundberg
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Chilotilapia rhoadesii, Escherichia coli, Homo sapiens
FEBS J.
276
1999-2011
2009
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3
-
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1
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7
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3
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3
6
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3
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3
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3
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1
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3
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3
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3
3
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711070
Matiollo
A transthyretin-related protei ...
Herbaspirillum seropedicae
Biochem. Biophys. Res. Commun.
387
712-716
2009
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1
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3
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2
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4
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1
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1
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1
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666761
Jung
Structural and functional anal ...
Bacillus subtilis
Proc. Natl. Acad. Sci. USA
103
9790-9795
2006
-
-
1
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3
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1
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1
4
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1
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1
1
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688334
Hennebry
The crystal structure of the t ...
Salmonella enterica subsp. enterica serovar Dublin
J. Mol. Biol.
359
1389-1399
2006
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-
1
1
3
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2
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6
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1
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1
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1
3
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3
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1
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1
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1
1
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688335
Zanotti
Structure of zebra fish HIUase ...
Danio rerio
J. Mol. Biol.
363
1-9
2006
-
-
1
1
1
-
-
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-
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2
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1
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1
1
2
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1
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1
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1
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1
1
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688940
Lee
Mouse transthyretin-related pr ...
Mus musculus
Mol. Cell
22
141-145
2006
-
-
1
-
6
-
-
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6
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1
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1
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5
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1
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6
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1
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1
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644454
Raychaudhuri
A familiar motif in a new cont ...
Glycine max
Biochemistry
42
6848-6852
2003
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1
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2
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2
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2
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2
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1
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4
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2
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1
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4
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2
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644455
Raychaudhuri
Cloning and expression of the ...
Glycine max
Plant Physiol.
130
2061-2068
2002
-
-
1
1
2
-
-
1
1
-
2
1
-
5
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1
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3
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2
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1
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1
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3
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1
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644456
Sarma
Identification and purificatio ...
Glycine max
J. Biol. Chem.
274
33863-33865
1999
-
-
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1
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1
1
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1
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1
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2
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