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Literature summary for 3.5.2.16 extracted from

  • Huang, C.Y.; Yang, Y.S.
    A novel cold-adapted imidase from fish Oreochromis niloticus that catalyzes hydrolysis of maleimide (2003), Biochem. Biophys. Res. Commun., 312, 467-472.
    View publication on PubMed
Search for more literature for 3.5.2.16

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 Phthalimide pH 7.9, 25°C Sus scrofa
1.6
-
 Phthalimide pH 7.9, 25°C Oreochromis niloticus
5.8
-
 Phthalimide pH 7.9, 25°C Agrobacterium tumefaciens
13
-
 Maleimide pH 7.9, 25°C Sus scrofa
180
-
 Maleimide pH 7.9, 25°C Agrobacterium tumefaciens
200
-
 Maleimide pH 7.9, 25°C Oreochromis niloticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oreochromis niloticus the enzyme plays a role in pyrimidine catabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
 NRRL B1, recombinant enzyme expressed in Escherichia coli
-
Oreochromis niloticus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oreochromis niloticus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-
liver
-
 Oreochromis niloticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
122
-
-
 Oreochromis niloticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maleimide + H2O
-
 Sus scrofa maleic acid monoamide
-
 ?
maleimide + H2O
-
 Agrobacterium tumefaciens maleic acid monoamide
-
 ?
maleimide + H2O
-
 Oreochromis niloticus maleic acid monoamide
-
 ?
additional information the enzyme plays a role in pyrimidine catabolism Oreochromis niloticus ?
-
 ?
phthalimide + H2O
-
 Sus scrofa 2-(aminocarbonyl)benzoic acid
-
 ?
phthalimide + H2O
-
 Agrobacterium tumefaciens 2-(aminocarbonyl)benzoic acid
-
 ?
phthalimide + H2O
-
 Oreochromis niloticus 2-(aminocarbonyl)benzoic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Oreochromis niloticus
70
-
 activity increases when temperature increases from 5 to 70°C Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 55 15°C: about 40% of maximal activity, 55°C: maximal activity, 65°C: no activity Oreochromis niloticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 60 min, about 10% loss of activity Sus scrofa
50
-
 10 min, about 25% loss of activity. 20 min, about 50% loss of activity. 30 min, about 60% loss of activity. 40 min, about 70% loss of activity. 50 min, about 80% loss of activity Oreochromis niloticus
50
-
 60 min, less than 5% loss of activity Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.67
-
 Phthalimide pH 7.9, 25°C Agrobacterium tumefaciens
25.17
-
 Phthalimide pH 7.9, 25°C Sus scrofa
106.7
-
 Maleimide pH 7.9, 25°C Sus scrofa
163.3
-
 Maleimide pH 7.9, 25°C Agrobacterium tumefaciens
428.3
-
 Phthalimide pH 7.9, 25°C Oreochromis niloticus
3950
-
 Maleimide pH 7.9, 25°C Oreochromis niloticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 40°C, half-life: 50 min Oreochromis niloticus
6
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Sus scrofa
6
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Agrobacterium tumefaciens
7
-
 40°C, half-life: 80 min Oreochromis niloticus
7
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Sus scrofa
7
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Agrobacterium tumefaciens
8
-
 40°C, half-life: 150 min Oreochromis niloticus
8
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Sus scrofa
8
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Agrobacterium tumefaciens
9
-
 40°C, half-life: 40 min Oreochromis niloticus
9
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Sus scrofa
9
-
 the stability decreases in the order pH 8, pH 7, pH 6, pH 9 Agrobacterium tumefaciens