Literature summary for 3.5.2.15 extracted from

Esquirol, L.; Peat, T.S.; Wilding, M.; Liu, J.W.; French, N.G.; Hartley, C.J.; Onagi, H.; Nebl, T.; Easton, C.J.; Newman, J.; Scott, C.
An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme (2018), J. Biol. Chem., 293, 7880-7891 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.35	-	cyanuric acid	pH 9, 22°C, purified from Pseudomonas sp. ADP	Pseudomonas sp. ADP

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. ADP	P58329	-	-
Pseudomonas sp. ADP DSM 11735	P58329	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas sp. ADP

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyanuric acid + H2O	-	Pseudomonas sp. ADP	1-carboxybiuret	-	?
cyanuric acid + H2O	-	Pseudomonas sp. ADP DSM 11735	1-carboxybiuret	-	?

Synonyms

Synonyms	Comment	Organism
AtzD	-	Pseudomonas sp. ADP

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
15.5	-	cyanuric acid	pH 9, 22°C, purified from Pseudomonas sp. ADP	Pseudomonas sp. ADP

General Information

General Information	Comment	Organism
metabolism	cyanuric acid is a metabolic intermediate of s-triazines, such as atrazine (a common herbicide) and melamine (used in resins and plastics). Cyanuric acid is mineralized to ammonia and carbon dioxide by the soil bacterium Pseudomonas sp. strain ADP via three hydrolytic enzymes , AtzD, AtzE, and AtzF	Pseudomonas sp. ADP

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
48	-	cyanuric acid	pH 9, 22°C, purified from Pseudomonas sp. ADP	Pseudomonas sp. ADP

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Release 2023.1 (January 2023)