Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoplasma acidophilum | |
NADP+ | - |
Thermoplasma acidophilum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Thermoplasma acidophilum |
Crystallization (Comment) | Organism |
---|---|
in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30600 | - |
SDS-PAGE | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | - |
strain DSM 1728 | - |
Purification (Comment) | Organism |
---|---|
His-Trap affinity column chromatography and Superdex-S200 gel filtration | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyltetrahydrofolate + H2O | - |
Thermoplasma acidophilum | methylenetetrahydrofolate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase | - |
Thermoplasma acidophilum |
MTHFDC | - |
Thermoplasma acidophilum |