Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.2.12 extracted from

  • Asano, Y.; Fukuta, Y.; Yoshida, Y.; Komeda, H.
    The screening, characterization, and use of omega-laurolactam hydrolase: a new enzymatic synthesis of 12-aminolauric acid (2008), Biosci. Biotechnol. Biochem., 72, 2141-2150.
    View publication on PubMed
Search for more literature for 3.5.2.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rhodococcus sp. U-224

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, complete enzyme inhibition Rhodococcus sp. U-224
p-chloromercuribenzoic acid 1 mM, complete enzyme inhibition Rhodococcus sp. U-224

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 SDS-PAGE Rhodococcus sp. U-224
79500
-
 gel chromatography Rhodococcus sp. U-224

Organism

Organism UniProt Comment Textmining
Acidovorax sp. T31 B7XBZ8
-
-
Cupriavidus sp. T7 B7XBZ7
-
-
Cupriavidus sp. U124 B7XBZ9
-
-
Rhodococcus sp. U-224 B5MF68
-
-
Sphingomonas sp. U238 B7XC00
-
-

Purification (Commentary)

Purification (Comment) Organism
90fold Rhodococcus sp. U-224

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.54
-
-
 Rhodococcus sp. U-224

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-amino-hexanoate-cyclic-dimer + H2O assay at pH 7.0, 30°C, 48 h Rhodococcus sp. U-224 ?
-
 ?
omega-laurolactam + H2O assay at pH 7.0, 30°C, 48 h Cupriavidus sp. U124 12-aminolauric acid
-
 ?
omega-laurolactam + H2O assay at pH 7.0, 30°C, 48 h Sphingomonas sp. U238 12-aminolauric acid
-
 ?
omega-laurolactam + H2O assay at pH 7.0, 30°C, 48 h Rhodococcus sp. U-224 12-aminolauric acid
-
 ?
omega-laurolactam + H2O assay at pH 7.0, 30°C, 48 h Cupriavidus sp. T7 12-aminolauric acid
-
 ?
omega-laurolactam + H2O assay at pH 7.0, 30°C, 48 h Acidovorax sp. T31 12-aminolauric acid
-
 ?
omega-octalactam + H2O assay at pH 7.0, 30°C, 48 h Rhodococcus sp. U-224 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Rhodococcus sp. U-224

Synonyms

Synonyms Comment Organism
omega-laurolactam hydrolase
-
 Cupriavidus sp. U124
omega-laurolactam hydrolase
-
 Sphingomonas sp. U238
omega-laurolactam hydrolase
-
 Rhodococcus sp. U-224
omega-laurolactam hydrolase
-
 Cupriavidus sp. T7
omega-laurolactam hydrolase
-
 Acidovorax sp. T31

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cupriavidus sp. U124
30
-
 assay at Sphingomonas sp. U238
30
-
 assay at Cupriavidus sp. T7
30
-
 assay at Acidovorax sp. T31
45
-
 pH around 7.2 in potassium phosphate buffer Rhodococcus sp. U-224

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 30 min, stable up to 35°C Rhodococcus sp. U-224
40
-
 30 min, 20% loss of activity Rhodococcus sp. U-224
45
-
 30 min, 80% loss of activity Rhodococcus sp. U-224
50
-
 30 min, completely inactivated Rhodococcus sp. U-224

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Cupriavidus sp. U124
7
-
 assay at Sphingomonas sp. U238
7
-
 assay at Cupriavidus sp. T7
7
-
 assay at Acidovorax sp. T31
7.2
-
 potassium phosphate buffer, 45°C Rhodococcus sp. U-224

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.1 8.2 30°C Rhodococcus sp. U-224