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Literature summary for 3.5.2.12 extracted from
- Characterization of the 6-aminohexanoate-dimer hydrolase from Pseudomonas sp. NK87 (1993), J. Gen. Microbiol., 139, 787-795.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropylphosphofluoridate | - |
Pseudomonas sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
1,8-Diazacyclotetradecane-2,9-dione | - |
Pseudomonas sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,8-diazacyclotetradecane-2,9-dione + H2O | Pseudomonas sp. | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
| 1,8-diazacyclotetradecane-2,9-dione + H2O | Pseudomonas sp. NK87 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
NK87 | - |
| Pseudomonas sp. NK87 | - |
NK87 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.004 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,8-diazacyclotetradecane-2,9-dione + H2O | - |
Pseudomonas sp. | N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
| 1,8-diazacyclotetradecane-2,9-dione + H2O | i.e. 6-aminohexanoic acid cyclic dimer | Pseudomonas sp. | N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
| 1,8-diazacyclotetradecane-2,9-dione + H2O | - |
Pseudomonas sp. NK87 | N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
- |
Pseudomonas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.7 | - |
1,8-Diazacyclotetradecane-2,9-dione | - |
Pseudomonas sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas sp. |
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