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Literature summary for 3.5.2.10 extracted from
- Expression of the creatininase gene from Pseudomonas putida RS65 in Escherichia coli (2000), J. Ind. Microbiol., 24, 2-6.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | analysis of serum creatinine concentration | Pseudomonas putida |
| medicine | diagnosis of renal, muscular and thyroid functions | Alcaligenes sp. |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned, sequenced and expressed in Escherichia coli DH5?, vector pCRN741, creatininase gene consists of 771 bp, encodes 257 amino acids, similarity of RS65 to PS-7 72.8% and Arthrobacter sp. TE1826 43.2% | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
- |
Alcaligenes sp. |
| 224000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcaligenes sp. | - |
- |
- |
| Pseudomonas putida | - |
NTU-8 and its creatininase producing mutant RS65 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, DEAE Sepharose CL-6B and Sepharose CL-6B column chromatographies, 50% total activity | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
by Escherichia coli transformants pCRN741 without and with IPTG 0.678 U per ml | Pseudomonas putida |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | dimer with a molecular weight of 175000 | Alcaligenes sp. |
| octamer | 8* 28000 | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudomonas putida |
| 45 | - |
- |
Alcaligenes sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
30 min, activity still 40% | Pseudomonas putida |
| 75 | - |
30 min, activity still 40% | Alcaligenes sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Pseudomonas putida |
| 7 | 8 | - |
Alcaligenes sp. |
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