BRENDA - Enzyme Database
show all sequences of 3.5.2.1

Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes

Hayaishi, O.; Kornberg, A.; J. Biol. Chem. 197, 717-732 (1952)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.37
-
Barbiturate
-
Mycobacterium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium sp.
-
-
-
Purification (Commentary)
Commentary
Organism
-
Mycobacterium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Mycobacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
barbiturate + H2O
-
209264
Mycobacterium sp.
malonate + urea
-
209264
Mycobacterium sp.
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Mycobacterium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
pH 7.5: about 70% of maximal activity, pH 9.5: about 45% of maximal activity
Mycobacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.37
-
Barbiturate
-
Mycobacterium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mycobacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Mycobacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
barbiturate + H2O
-
209264
Mycobacterium sp.
malonate + urea
-
209264
Mycobacterium sp.
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Mycobacterium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
pH 7.5: about 70% of maximal activity, pH 9.5: about 45% of maximal activity
Mycobacterium sp.
Other publictions for EC 3.5.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
652350
Soong
Barbiturase, a novel zinc-cont ...
Rhodococcus erythropolis
J. Biol. Chem.
277
7051-7058
2002
-
-
1
-
-
-
17
1
-
1
2
-
-
3
-
-
1
-
-
-
-
2
2
2
-
-
1
-
1
-
-
-
3
-
-
-
-
1
-
-
-
-
-
17
3
1
-
1
2
-
-
-
-
1
-
-
-
2
2
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
209263
Patel
-
Oxidative catabolism of uracil ...
Klebsiella aerogenes
FEMS Microbiol. Lett.
40
33-36
1987
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209264
Hayaishi
Metabolism of cytosine, thymin ...
Mycobacterium sp.
J. Biol. Chem.
197
717-732
1952
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-