Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 25% inhibition at 0.1 mM | Arabidopsis thaliana | |
8-Hydroxyquinoline-5-sulfonate | complete inhibition at 1 mM | Arabidopsis thaliana | |
Cu2+ | complete inhibition | Arabidopsis thaliana | |
DTNB | 25% inhibition at 1 mM | Arabidopsis thaliana | |
DTT | 53% inhibition at 1 mM | Arabidopsis thaliana | |
EDTA | 21% inhibition at 1 mM | Arabidopsis thaliana | |
folate | substrate inhibition, competitive | Arabidopsis thaliana | |
Hg2+ | complete inhibition | Arabidopsis thaliana | |
additional information | the enzyme activity is fairly insensitive to serine, cysteine, and aspartic reagents, but is inhibited by metal chelators | Arabidopsis thaliana | |
N,N,N',N'-tetrakis[2-pyridylmethyl]ethylenediamine | i.e. TPEN, 53% inhibition at 0.1 mM | Arabidopsis thaliana | |
pepstatin | 25% inhibition at 0.0015 mM | Arabidopsis thaliana | |
PMSF | 15% inhibition at 1 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana | |
0.37 | - |
4-aminobenzoylglutamate | pH 7.4, 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pisum sativum | 5829 | - |
mitochondrion | - |
Pisum sativum | 5739 | - |
additional information | no activity in chloroplasts, the enzyme is probably also partially located in vacuoles | Pisum sativum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 54% activation at 0.1 mM, metalloenzyme | Arabidopsis thaliana | |
additional information | metalloenzyme | Spinacia oleracea | |
additional information | metalloenzyme | Pisum sativum | |
additional information | metalloenzyme | Zea mays | |
additional information | metalloenzyme | Solanum lycopersicum | |
additional information | no activation by Zn2+ and Ni2+ | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
main peak, gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobenzoylglutamate + H2O | Spinacia oleracea | the enzyme is involved in the folate salvage pathway, overview | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | Pisum sativum | the enzyme is involved in the folate salvage pathway, overview | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | Zea mays | the enzyme is involved in the folate salvage pathway, overview | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | Arabidopsis thaliana | the enzyme is involved in the folate salvage pathway, overview | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | Solanum lycopersicum | the enzyme is involved in the folate salvage pathway, overview | 4-aminobenzoic acid + L-glutamate | - |
? | |
folic acid + H2O | Spinacia oleracea | - |
pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | Pisum sativum | - |
pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | Zea mays | - |
pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | Arabidopsis thaliana | - |
pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | Solanum lycopersicum | - |
pteroic acid + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Pisum sativum | - |
cv. Laxtons Progress 9 | - |
Solanum lycopersicum | - |
cv. Micro-Tom | - |
Spinacia oleracea | - |
- |
- |
Zea mays | - |
cv. NK508 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from 268fold leaves and 86fold from roots by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, followed by gel filtration, activity loss during purification exceeds 95% | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N2-(4-aminobenzoyl)-L-glutamate + H2O = 4-aminobenzoate + L-glutamate | distinct from carboxypeptidase G, EC 3.4.17.11, and glutamate carboxypeptidase II, EC 3.4.17.21 | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
leaf | - |
Spinacia oleracea | - |
leaf | - |
Pisum sativum | - |
leaf | - |
Zea mays | - |
leaf | - |
Arabidopsis thaliana | - |
leaf | - |
Solanum lycopersicum | - |
mesophyll | - |
Pisum sativum | - |
root | high enzyme level, several fold higher than in leaves | Spinacia oleracea | - |
root | high enzyme level, several fold higher than in leaves | Pisum sativum | - |
root | high enzyme level, several fold higher than in leaves | Zea mays | - |
root | high enzyme level, several fold higher than in leaves | Solanum lycopersicum | - |
root | in vitro tissue culture, high enzyme level, several fold higher than in leaves | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme shows very low activity and is a rare enzyme | Spinacia oleracea |
additional information | - |
the enzyme shows very low activity and is a rare enzyme | Pisum sativum |
additional information | - |
the enzyme shows very low activity and is a rare enzyme | Zea mays |
additional information | - |
the enzyme shows very low activity and is a rare enzyme | Arabidopsis thaliana |
additional information | - |
the enzyme shows very low activity and is a rare enzyme | Solanum lycopersicum |
0.00029 | - |
purified enzyme from roots | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobenzoylglutamate + H2O | - |
Spinacia oleracea | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | - |
Pisum sativum | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | - |
Zea mays | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | - |
Arabidopsis thaliana | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | - |
Solanum lycopersicum | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | the enzyme is involved in the folate salvage pathway, overview | Spinacia oleracea | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | the enzyme is involved in the folate salvage pathway, overview | Pisum sativum | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | the enzyme is involved in the folate salvage pathway, overview | Zea mays | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | the enzyme is involved in the folate salvage pathway, overview | Arabidopsis thaliana | 4-aminobenzoic acid + L-glutamate | - |
? | |
4-aminobenzoylglutamate + H2O | the enzyme is involved in the folate salvage pathway, overview | Solanum lycopersicum | 4-aminobenzoic acid + L-glutamate | - |
? | |
folic acid + H2O | - |
Spinacia oleracea | pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | - |
Pisum sativum | pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | - |
Zea mays | pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | - |
Arabidopsis thaliana | pteroic acid + L-glutamate | - |
? | |
folic acid + H2O | - |
Solanum lycopersicum | pteroic acid + L-glutamate | - |
? | |
additional information | the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview | Spinacia oleracea | ? | - |
? | |
additional information | the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview | Pisum sativum | ? | - |
? | |
additional information | the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview | Zea mays | ? | - |
? | |
additional information | the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview | Solanum lycopersicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
p-aminobenzoylglutamate hydrolase | - |
Spinacia oleracea |
p-aminobenzoylglutamate hydrolase | - |
Pisum sativum |
p-aminobenzoylglutamate hydrolase | - |
Zea mays |
p-aminobenzoylglutamate hydrolase | - |
Arabidopsis thaliana |
p-aminobenzoylglutamate hydrolase | - |
Solanum lycopersicum |
pABAGlu hydrolase | - |
Spinacia oleracea |
pABAGlu hydrolase | - |
Pisum sativum |
pABAGlu hydrolase | - |
Zea mays |
pABAGlu hydrolase | - |
Arabidopsis thaliana |
pABAGlu hydrolase | - |
Solanum lycopersicum |
PGH | - |
Spinacia oleracea |
PGH | - |
Pisum sativum |
PGH | - |
Zea mays |
PGH | - |
Arabidopsis thaliana |
PGH | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spinacia oleracea |
37 | - |
assay at | Pisum sativum |
37 | - |
assay at | Zea mays |
37 | - |
assay at | Arabidopsis thaliana |
37 | - |
assay at | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.5 | - |
Arabidopsis thaliana |
7.4 | - |
assay at | Spinacia oleracea |
7.4 | - |
assay at | Pisum sativum |
7.4 | - |
assay at | Zea mays |
7.4 | - |
assay at | Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.3 | 50% of maximal activity at pH 6.5 and pH 8.3, pH profile | Arabidopsis thaliana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.052 | - |
pH 7.4, 37°C, versus 4-aminobenzoylglutamate | Arabidopsis thaliana | folic acid |