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Literature summary for 3.5.1.B18 extracted from

  • Bozzo, G.G.; Basset, G.J.; Naponelli, V.; Noiriel, A.; Gregory, J.F.; Hanson, A.D.
    Characterization of the folate salvage enzyme p-aminobenzoylglutamate hydrolase in plants (2008), Phytochemistry, 69, 29-37.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 25% inhibition at 0.1 mM Arabidopsis thaliana
8-Hydroxyquinoline-5-sulfonate complete inhibition at 1 mM Arabidopsis thaliana
Cu2+ complete inhibition Arabidopsis thaliana
DTNB 25% inhibition at 1 mM Arabidopsis thaliana
DTT 53% inhibition at 1 mM Arabidopsis thaliana
EDTA 21% inhibition at 1 mM Arabidopsis thaliana
folate substrate inhibition, competitive Arabidopsis thaliana
Hg2+ complete inhibition Arabidopsis thaliana
additional information the enzyme activity is fairly insensitive to serine, cysteine, and aspartic reagents, but is inhibited by metal chelators Arabidopsis thaliana
N,N,N',N'-tetrakis[2-pyridylmethyl]ethylenediamine i.e. TPEN, 53% inhibition at 0.1 mM Arabidopsis thaliana
pepstatin 25% inhibition at 0.0015 mM Arabidopsis thaliana
PMSF 15% inhibition at 1 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Arabidopsis thaliana
0.37
-
4-aminobenzoylglutamate pH 7.4, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Pisum sativum 5829
-
mitochondrion
-
Pisum sativum 5739
-
additional information no activity in chloroplasts, the enzyme is probably also partially located in vacuoles Pisum sativum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 54% activation at 0.1 mM, metalloenzyme Arabidopsis thaliana
additional information metalloenzyme Spinacia oleracea
additional information metalloenzyme Pisum sativum
additional information metalloenzyme Zea mays
additional information metalloenzyme Solanum lycopersicum
additional information no activation by Zn2+ and Ni2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
main peak, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobenzoylglutamate + H2O Spinacia oleracea the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O Pisum sativum the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O Zea mays the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O Arabidopsis thaliana the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O Solanum lycopersicum the enzyme is involved in the folate salvage pathway, overview 4-aminobenzoic acid + L-glutamate
-
?
folic acid + H2O Spinacia oleracea
-
pteroic acid + L-glutamate
-
?
folic acid + H2O Pisum sativum
-
pteroic acid + L-glutamate
-
?
folic acid + H2O Zea mays
-
pteroic acid + L-glutamate
-
?
folic acid + H2O Arabidopsis thaliana
-
pteroic acid + L-glutamate
-
?
folic acid + H2O Solanum lycopersicum
-
pteroic acid + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Columbia
-
Pisum sativum
-
cv. Laxton’s Progress 9
-
Solanum lycopersicum
-
cv. Micro-Tom
-
Spinacia oleracea
-
-
-
Zea mays
-
cv. NK508
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from 268fold leaves and 86fold from roots by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, followed by gel filtration, activity loss during purification exceeds 95% Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
N2-(4-aminobenzoyl)-L-glutamate + H2O = 4-aminobenzoate + L-glutamate distinct from carboxypeptidase G, EC 3.4.17.11, and glutamate carboxypeptidase II, EC 3.4.17.21 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Solanum lycopersicum
-
leaf
-
Spinacia oleracea
-
leaf
-
Pisum sativum
-
leaf
-
Zea mays
-
leaf
-
Arabidopsis thaliana
-
leaf
-
Solanum lycopersicum
-
mesophyll
-
Pisum sativum
-
root high enzyme level, several fold higher than in leaves Spinacia oleracea
-
root high enzyme level, several fold higher than in leaves Pisum sativum
-
root high enzyme level, several fold higher than in leaves Zea mays
-
root high enzyme level, several fold higher than in leaves Solanum lycopersicum
-
root in vitro tissue culture, high enzyme level, several fold higher than in leaves Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the enzyme shows very low activity and is a rare enzyme Spinacia oleracea
additional information
-
the enzyme shows very low activity and is a rare enzyme Pisum sativum
additional information
-
the enzyme shows very low activity and is a rare enzyme Zea mays
additional information
-
the enzyme shows very low activity and is a rare enzyme Arabidopsis thaliana
additional information
-
the enzyme shows very low activity and is a rare enzyme Solanum lycopersicum
0.00029
-
purified enzyme from roots Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobenzoylglutamate + H2O
-
Spinacia oleracea 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O
-
Pisum sativum 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O
-
Zea mays 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O
-
Arabidopsis thaliana 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O
-
Solanum lycopersicum 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Spinacia oleracea 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Pisum sativum 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Zea mays 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Arabidopsis thaliana 4-aminobenzoic acid + L-glutamate
-
?
4-aminobenzoylglutamate + H2O the enzyme is involved in the folate salvage pathway, overview Solanum lycopersicum 4-aminobenzoic acid + L-glutamate
-
?
folic acid + H2O
-
Spinacia oleracea pteroic acid + L-glutamate
-
?
folic acid + H2O
-
Pisum sativum pteroic acid + L-glutamate
-
?
folic acid + H2O
-
Zea mays pteroic acid + L-glutamate
-
?
folic acid + H2O
-
Arabidopsis thaliana pteroic acid + L-glutamate
-
?
folic acid + H2O
-
Solanum lycopersicum pteroic acid + L-glutamate
-
?
additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Spinacia oleracea ?
-
?
additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Pisum sativum ?
-
?
additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Zea mays ?
-
?
additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Arabidopsis thaliana ?
-
?
additional information the enzyme is distinct from carboxypeptidase G, EC 3.4.17.11, which also shows p-aminobenzoylglutamate hydrolase activity, overview Solanum lycopersicum ?
-
?

Subunits

Subunits Comment Organism
oligomer
-
Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
p-aminobenzoylglutamate hydrolase
-
Spinacia oleracea
p-aminobenzoylglutamate hydrolase
-
Pisum sativum
p-aminobenzoylglutamate hydrolase
-
Zea mays
p-aminobenzoylglutamate hydrolase
-
Arabidopsis thaliana
p-aminobenzoylglutamate hydrolase
-
Solanum lycopersicum
pABAGlu hydrolase
-
Spinacia oleracea
pABAGlu hydrolase
-
Pisum sativum
pABAGlu hydrolase
-
Zea mays
pABAGlu hydrolase
-
Arabidopsis thaliana
pABAGlu hydrolase
-
Solanum lycopersicum
PGH
-
Spinacia oleracea
PGH
-
Pisum sativum
PGH
-
Zea mays
PGH
-
Arabidopsis thaliana
PGH
-
Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Spinacia oleracea
37
-
assay at Pisum sativum
37
-
assay at Zea mays
37
-
assay at Arabidopsis thaliana
37
-
assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.5
-
Arabidopsis thaliana
7.4
-
assay at Spinacia oleracea
7.4
-
assay at Pisum sativum
7.4
-
assay at Zea mays
7.4
-
assay at Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.3 50% of maximal activity at pH 6.5 and pH 8.3, pH profile Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.052
-
pH 7.4, 37°C, versus 4-aminobenzoylglutamate Arabidopsis thaliana folic acid