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Literature summary for 3.5.1.B15 extracted from

  • Du, X.; Wang, W.; Kim, R.; Yakota, H.; Nguyen, H.; Kim, S.H.
    Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii (2001), Biochemistry, 40, 14166-14172 .
    View publication on PubMed
Search for more literature for 3.5.1.B15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a methionine auxotroph, Escherichia coli strain B834 Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
three-dimensional structure of pyrazinamidase at 2.05 A resolution and the structure of its complex with Zn2+ at 1.65 A resolution Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+ increases the kcat of the enzyme by about 19-fold. fold. Other divalent ions, including Mg2+, Mn2+, Ca2+, and Cd2+, do not increase the activity. A metal ion-binding site is revealed in the active site and confirmed by crystal structure of the enzyme in complex with Zn2+ Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58727
-
-
Pyrococcus horikoshii OT-3 O58727
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.3
-
 22°C, pH 6.5, without Zn2+ Pyrococcus horikoshii
333
-
 22°C, pH 6.5, in the presence of 2 mM Zn2+ Pyrococcus horikoshii
357
-
 60°C, pH 6.5, without Zn2+ Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyrazinamide + H2O
-
 Pyrococcus horikoshii pyrazinoic acid + NH3
-
 ?
pyrazinamide + H2O
-
 Pyrococcus horikoshii OT-3 pyrazinoic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PH0999
-
 Pyrococcus horikoshii
PZAase
-
 Pyrococcus horikoshii